1x75: Difference between revisions

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-[[Image:1x75.gif|left|200px]]
-<!--
+==CcdB:GyrA14 complex==
-The line below this paragraph, containing "STRUCTURE_1x75", creates the "Structure Box" on the page.
+<StructureSection load='1x75' size='340' side='right'caption='[[1x75]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1x75]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X75 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x75 OCA], [https://pdbe.org/1x75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x75 RCSB], [https://www.ebi.ac.uk/pdbsum/1x75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x75 ProSAT]</span></td></tr>
-{{STRUCTURE_1x75|  PDB=1x75  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GYRA_ECOLI GYRA_ECOLI] DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.<ref>PMID:12051842</ref> <ref>PMID:18642932</ref> <ref>PMID:19965760</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x75_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x75 ConSurf].
+<div style="clear:both"></div>
-'''CcdB:GyrA14 complex'''
+==See Also==
+*[[Gyrase 3D Structures|Gyrase 3D Structures]]
+== References ==
-==Overview==
+<references/>
-Gyrase is an ubiquitous bacterial enzyme that is responsible for disentangling DNA during DNA replication and transcription. It is the target of the toxin CcdB, a paradigm for plasmid addiction systems and related bacterial toxin-antitoxin systems. The crystal structure of CcdB and the dimerization domain of the A subunit of gyrase (GyrA14) dictates an open conformation for the catalytic domain of gyrase when CcdB is bound. The action of CcdB is one of a wedge that stabilizes a dead-end covalent gyrase:DNA adduct. Although CcdB and GyrA14 form a globally symmetric complex where the two 2-fold axes of both dimers align, the complex is asymmetric in its details. At the centre of the interaction site, the Trp99 pair of CcdB stacks with the Arg462 pair of GyrA14, explaining why the Arg462Cys mutation in the A subunit of gyrase confers resistance to CcdB. Overexpression of GyrA14 protects Escherichia coli cells against CcdB, mimicking the action of the antidote CcdA.
+__TOC__
+</StructureSection>
-==About this Structure==
-X75 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X75 OCA].
-==Reference==
-Molecular basis of gyrase poisoning by the addiction toxin CcdB., Dao-Thi MH, Van Melderen L, De Genst E, Afif H, Buts L, Wyns L, Loris R, J Mol Biol. 2005 May 20;348(5):1091-102. Epub 2005 Apr 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15854646 15854646]
 [[Category: Escherichia coli]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Dao-Thi, M H.]]
+[[Category: Dao-Thi MH]]
-[[Category: Genst, E De.]]
+[[Category: De Genst E]]
-[[Category: Loris, R.]]
+[[Category: Loris R]]
-[[Category: Melderen, L Van.]]
+[[Category: Van Melderen L]]
-[[Category: Wyns, L.]]
+[[Category: Wyns L]]
-[[Category: Bacterial cell death]]
-[[Category: Ccdb]]
-[[Category: Gyrase]]
-[[Category: Plasmid addiction]]
-[[Category: Ta system]]
-[[Category: Topoisomerase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 14:39:04 2008''