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[[Image:1x0g.gif|left|200px]]


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==Crystal Structure of IscA with the [2Fe-2S] cluster==
The line below this paragraph, containing "STRUCTURE_1x0g", creates the "Structure Box" on the page.
<StructureSection load='1x0g' size='340' side='right'caption='[[1x0g]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1x0g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X0G FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
{{STRUCTURE_1x0g|  PDB=1x0g  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x0g OCA], [https://pdbe.org/1x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x0g RCSB], [https://www.ebi.ac.uk/pdbsum/1x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x0g ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure of IscA with the [2Fe-2S] cluster'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q8DLM0_THEVB Q8DLM0_THEVB]  
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
It has been shown that the so-called scaffold proteins are vital in Fe-S cluster biosynthesis by providing an intermediate site for the assembly of Fe-S clusters. However, since no structural information on such scaffold proteins with bound Fe-S cluster intermediates is available, the structural basis of the core of Fe-S cluster biosynthesis remains poorly understood. Here we report the first Fe-S cluster-bound crystal structure of a scaffold protein, IscA, from Thermosynechococcus elongatus, which carries three strictly conserved cysteine residues. Surprisingly, one partially exposed [2Fe-2S] cluster is coordinated by two conformationally distinct IscA protomers, termed alpha and beta, with asymmetric cysteinyl ligation by Cys37, Cys101, Cys103 from alpha and Cys103 from beta. In the crystal, two alphabeta dimers form an unusual domain-swapped tetramer via central domains of beta protomers. Together with additional biochemical data supporting its physiologically relevant configuration, we propose that the unique asymmetric Fe-S cluster coordination and the resulting distinct conformational stabilities of the two IscA protomers are central to the function of IscA-type Fe-S cluster biosynthetic scaffold.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x0/1x0g_consurf.spt"</scriptWhenChecked>
1X0G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X0G OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold., Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M, J Mol Biol. 2006 Jun 30;360(1):117-32. Epub 2006 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16730357 16730357]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x0g ConSurf].
[[Category: Bacteria]]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: Kondou, Y.]]
</StructureSection>
[[Category: Lee, S J.]]
[[Category: Large Structures]]
[[Category: Morimoto, K.]]
[[Category: Thermosynechococcus vestitus BP-1]]
[[Category: Nakai, M.]]
[[Category: Kondou Y]]
[[Category: Tsukihara, T.]]
[[Category: Lee SJ]]
[[Category: Yamashita, E.]]
[[Category: Morimoto K]]
[[Category: Biosynthesis]]
[[Category: Nakai M]]
[[Category: Cyanobacteria]]
[[Category: Tsukihara T]]
[[Category: Domain swapping]]
[[Category: Yamashita E]]
[[Category: Fe-s cluster]]
[[Category: Iron]]
[[Category: Iron-sulfur cluster protein]]
[[Category: Isc]]
[[Category: Isca]]
[[Category: Scaffold]]
[[Category: Sulfur]]
[[Category: Tetrameric]]
[[Category: Three conserved cy]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 14:22:31 2008''

Latest revision as of 16:35, 13 March 2024

Crystal Structure of IscA with the [2Fe-2S] clusterCrystal Structure of IscA with the [2Fe-2S] cluster

Structural highlights

1x0g is a 4 chain structure with sequence from Thermosynechococcus vestitus BP-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8DLM0_THEVB

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1x0g, resolution 2.50Å

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OCA
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