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| [[Image:1x0g.gif|left|200px]]
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| {{Structure
| | ==Crystal Structure of IscA with the [2Fe-2S] cluster== |
| |PDB= 1x0g |SIZE=350|CAPTION= <scene name='initialview01'>1x0g</scene>, resolution 2.50Å
| | <StructureSection load='1x0g' size='340' side='right'caption='[[1x0g]], [[Resolution|resolution]] 2.50Å' scene=''> |
| |SITE= | | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
| | <table><tr><td colspan='2'>[[1x0g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X0G FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| |GENE= tll0464 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x0g OCA], [https://pdbe.org/1x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x0g RCSB], [https://www.ebi.ac.uk/pdbsum/1x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x0g ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x0g OCA], [http://www.ebi.ac.uk/pdbsum/1x0g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x0g RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/Q8DLM0_THEVB Q8DLM0_THEVB] |
| | | == Evolutionary Conservation == |
| '''Crystal Structure of IscA with the [2Fe-2S] cluster'''
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | | Check<jmol> |
| | | <jmolCheckbox> |
| ==Overview== | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x0/1x0g_consurf.spt"</scriptWhenChecked> |
| It has been shown that the so-called scaffold proteins are vital in Fe-S cluster biosynthesis by providing an intermediate site for the assembly of Fe-S clusters. However, since no structural information on such scaffold proteins with bound Fe-S cluster intermediates is available, the structural basis of the core of Fe-S cluster biosynthesis remains poorly understood. Here we report the first Fe-S cluster-bound crystal structure of a scaffold protein, IscA, from Thermosynechococcus elongatus, which carries three strictly conserved cysteine residues. Surprisingly, one partially exposed [2Fe-2S] cluster is coordinated by two conformationally distinct IscA protomers, termed alpha and beta, with asymmetric cysteinyl ligation by Cys37, Cys101, Cys103 from alpha and Cys103 from beta. In the crystal, two alphabeta dimers form an unusual domain-swapped tetramer via central domains of beta protomers. Together with additional biochemical data supporting its physiologically relevant configuration, we propose that the unique asymmetric Fe-S cluster coordination and the resulting distinct conformational stabilities of the two IscA protomers are central to the function of IscA-type Fe-S cluster biosynthetic scaffold.
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | | <text>to colour the structure by Evolutionary Conservation</text> |
| ==About this Structure== | | </jmolCheckbox> |
| 1X0G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X0G OCA].
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x0g ConSurf]. |
| | | <div style="clear:both"></div> |
| ==Reference==
| | __TOC__ |
| The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold., Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M, J Mol Biol. 2006 Jun 30;360(1):117-32. Epub 2006 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16730357 16730357]
| | </StructureSection> |
| [[Category: Bacteria]] | | [[Category: Large Structures]] |
| [[Category: Single protein]] | | [[Category: Thermosynechococcus vestitus BP-1]] |
| [[Category: Kondou, Y.]] | | [[Category: Kondou Y]] |
| [[Category: Lee, S J.]] | | [[Category: Lee SJ]] |
| [[Category: Morimoto, K.]] | | [[Category: Morimoto K]] |
| [[Category: Nakai, M.]] | | [[Category: Nakai M]] |
| [[Category: Tsukihara, T.]] | | [[Category: Tsukihara T]] |
| [[Category: Yamashita, E.]] | | [[Category: Yamashita E]] |
| [[Category: [2fe-2s]]]
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| [[Category: biosynthesis]]
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| [[Category: cyanobacteria]]
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| [[Category: domain swapping]]
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| [[Category: fe-s cluster]]
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| [[Category: iron]]
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| [[Category: iron-sulfur cluster protein]]
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| [[Category: isc]]
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| [[Category: isca]]
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| [[Category: scaffold]]
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| [[Category: sulfur]]
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| [[Category: tetrameric]]
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| [[Category: three conserved cy]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:43:17 2008''
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