1wwh: Difference between revisions

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 <StructureSection load='1wwh' size='340' side='right'caption='[[1wwh]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1wwh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WWH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WWH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1wwh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WWH FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wwh OCA], [http://pdbe.org/1wwh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wwh RCSB], [http://www.ebi.ac.uk/pdbsum/1wwh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wwh ProSAT], [http://www.topsan.org/Proteins/RSGI/1wwh TOPSAN]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wwh OCA], [https://pdbe.org/1wwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wwh RCSB], [https://www.ebi.ac.uk/pdbsum/1wwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wwh ProSAT], [https://www.topsan.org/Proteins/RSGI/1wwh TOPSAN]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NUP53_MOUSE NUP53_MOUSE]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC (By similarity).
+[https://www.uniprot.org/uniprot/NUP35_MOUSE NUP35_MOUSE] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
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 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wwh ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-The nuclear pore complex mediates the transport of macromolecules across the nuclear envelope (NE). The vertebrate nuclear pore protein Nup35, the ortholog of Saccharomyces cerevisiae Nup53p, is suggested to interact with the NE membrane and to be required for nuclear morphology. The highly conserved region between vertebrate Nup35 and yeast Nup53p is predicted to contain an RNA-recognition motif (RRM) domain. Due to its low level of sequence homology with other RRM domains, the RNP1 and RNP2 motifs have not been identified in its primary structure. In the present study, we solved the crystal structure of the RRM domain of mouse Nup35 at 2.7 A resolution. The Nup35 RRM domain monomer adopts the characteristic betaalphabetabetaalphabeta topology, as in other reported RRM domains. The structure allowed us to locate the atypical RNP1 and RNP2 motifs. Among the RNP motif residues, those on the beta-sheet surface are different from those of the canonical RRM domains, while those buried in the hydrophobic core are highly conserved. The RRM domain forms a homodimer in the crystal, in accordance with analytical ultracentrifugation experiments. The beta-sheet surface of the RRM domain, with its atypical RNP motifs, contributes to homodimerization mainly by hydrophobic interactions: the side-chain of Met236 in the beta4 strand of one Nup35 molecule is sandwiched by the aromatic side-chains of Phe178 in the beta1 strand and Trp209 in the beta3 strand of the other Nup35 molecule in the dimer. This structure reveals a new homodimerization mode of the RRM domain.
-The crystal structure of mouse Nup35 reveals atypical RNP motifs and novel homodimerization of the RRM domain.,Handa N, Kukimoto-Niino M, Akasaka R, Kishishita S, Murayama K, Terada T, Inoue M, Kigawa T, Kose S, Imamoto N, Tanaka A, Hayashizaki Y, Shirouzu M, Yokoyama S J Mol Biol. 2006 Oct 13;363(1):114-24. Epub 2006 Aug 3. PMID:16962612<ref>PMID:16962612</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 1wwh" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Hamana, H]]
+[[Category: Hamana H]]
-[[Category: Handa, N]]
+[[Category: Handa N]]
-[[Category: Kukimoto, M]]
+[[Category: Kukimoto M]]
-[[Category: Murayama, K]]
+[[Category: Murayama K]]
-[[Category: Structural genomic]]
+[[Category: Shirouzu M]]
-[[Category: Shirouzu, M]]
+[[Category: Takemoto C]]
-[[Category: Takemoto, C]]
+[[Category: Terada T]]
-[[Category: Terada, T]]
+[[Category: Uchikubo T]]
-[[Category: Uchikubo, T]]
+[[Category: Yokoyama S]]
-[[Category: Yokoyama, S]]
-[[Category: Mppn]]
-[[Category: Protein transport]]
-[[Category: Rsgi]]