1wu3: Difference between revisions

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-[[Image:1wu3.jpg|left|200px]]<br /><applet load="1wu3" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1wu3, resolution 2.15&Aring;" />
-'''Crystal structure of recombinant murine interferon beta'''<br />
-==Overview==
+==Crystal structure of recombinant murine interferon beta==
-The crystal structure of recombinant murine interferon-beta (reMuIFN-beta) has been refined at 2.15 A resolution using newly collected synchrotron data. Based on 11,228 reflections (8.0 to 2.15 A), a final R-factor of 19.1% (with a free R-factor of 25.8%) was obtained with a model obeying standard geometry within 0.013 A in bond lengths and 1.4 degrees in bond angles. Compared with the previously reported model, several amino acid residues in helix A are frame-shifted, the conformations are changed for parts of loops AB and BC, helix C is extended and a new short helix exists in loop CD. Evolutionary considerations taken together, the type I interferons appear to share common structural features with respect to the chain-folding topology and the hydrogen-bond networks between various polypeptide segments. Specifically, the disposition of the C-terminal segment of loop AB (after Arg33), known to be an important receptor-binding site, seems to be strictly maintained among the type I interferons. The exposed amino acid residues on helices A and C, which have recently been implicated as the binding site for another receptor molecule, are less well conserved. This may be responsible for varied cellular effects among the subtypes of type I interferons.
+<StructureSection load='1wu3' size='340' side='right'caption='[[1wu3]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1wu3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rmi 1rmi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WU3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wu3 OCA], [https://pdbe.org/1wu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wu3 RCSB], [https://www.ebi.ac.uk/pdbsum/1wu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wu3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IFNB_MOUSE IFNB_MOUSE] Has antiviral, antibacterial and anticancer activities.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wu/1wu3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wu3 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-WU3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry 1RMI. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WU3 OCA].
+*[[Interferon 3D structures|Interferon 3D structures]]
+*[[Multiple sclerosis|Multiple sclerosis]]
-==Reference==
+__TOC__
-Refined crystal structure of recombinant murine interferon-beta at 2.15 A resolution., Senda T, Saitoh S, Mitsui Y, J Mol Biol. 1995 Oct 13;253(1):187-207. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7473712 7473712]
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Single protein]]
+[[Category: Mitsui Y]]
-[[Category: Mitsui, Y.]]
+[[Category: Saitoh S]]
-[[Category: Saitoh, S.]]
+[[Category: Senda T]]
-[[Category: Senda, T.]]
-[[Category: alpha-helix-bundle]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:48:09 2008''