1wqh: Difference between revisions

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New page: left|200px<br /><applet load="1wqh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wqh, resolution 2.90Å" /> '''Crystal structure of...
 
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[[Image:1wqh.gif|left|200px]]<br /><applet load="1wqh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1wqh, resolution 2.90&Aring;" />
'''Crystal structure of ribosome recycling factor from Mycobacterium tuberculosis'''<br />


==Overview==
==Crystal structure of ribosome recycling factor from Mycobacterium tuberculosis==
The crystal structure of Mycobacterium tuberculosis ribosome recycling, factor has been determined and refined against three X-ray diffraction, data sets, two collected at room temperature and the other at 100K. The, two room-temperature data sets differ in the radiation damage suffered by, the crystals before the data used for processing were collected. A, comparison between the structures refined against the two data sets, indicates the possibility of radiation-induced conformational change. The, L-shaped molecule is composed of a long three-helix bundle domain (domain, I) and a globular domain (domain II) connected by a linker region. The, main difference between the room-temperature structure and the low, temperature structure is in the rotation of domain II about an axis close, to its libration axis. This observation and a detailed comparative study, of ribosome recycling factors (RRFs) of known structures led to an, elaboration of the present understanding of the structural variability of, RRF. The variability involves a change in the angle between the two arms, of the molecule, a rotation of domain II in a plane nearly perpendicular, to the axis of the helix bundle and an internal rotation of domain II., Furthermore, the domains and the linker could be delineated into fixed and, variable regions in a physically meaningful manner. The relative mobility, of the domains of the molecule in the crystal structure appears to be, similar to that in the ribosome--RRF complex. That permits a meaningful, discussion of the structural features of RRF in terms of ribosome--RRF, interactions. The structure also provides insights into the results of, inter-species complementation studies.
<StructureSection load='1wqh' size='340' side='right'caption='[[1wqh]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wqh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WQH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wqh OCA], [https://pdbe.org/1wqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wqh RCSB], [https://www.ebi.ac.uk/pdbsum/1wqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wqh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RRF_MYCTU RRF_MYCTU] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/1wqh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wqh ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1WQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WQH OCA].
*[[Ribosome recycling factor|Ribosome recycling factor]]
 
__TOC__
==Reference==
</StructureSection>
X-ray structural studies of Mycobacterium tuberculosis RRF and a comparative study of RRFs of known structure. Molecular plasticity and biological implications., Saikrishnan K, Kalapala SK, Varshney U, Vijayan M, J Mol Biol. 2005 Jan 7;345(1):29-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15567408 15567408]
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Kalapala SK]]
[[Category: Kalapala, S.K.]]
[[Category: Saikrishnan K]]
[[Category: Saikrishnan, K.]]
[[Category: Varshney U]]
[[Category: Varshney, U.]]
[[Category: Vijayan M]]
[[Category: Vijayan, M.]]
[[Category: CD]]
[[Category: protein synthesis]]
[[Category: translation factor]]
[[Category: triple-helix bundle]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:36:36 2007''

Latest revision as of 16:33, 13 March 2024

Crystal structure of ribosome recycling factor from Mycobacterium tuberculosisCrystal structure of ribosome recycling factor from Mycobacterium tuberculosis

Structural highlights

1wqh is a 1 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.9Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RRF_MYCTU Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1wqh, resolution 2.90Å

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