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| [[Image:1woj.gif|left|200px]]
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| {{Structure
| | ==Crystal structure of human phosphodiesterase== |
| |PDB= 1woj |SIZE=350|CAPTION= <scene name='initialview01'>1woj</scene>, resolution 1.80Å
| | <StructureSection load='1woj' size='340' side='right'caption='[[1woj]], [[Resolution|resolution]] 1.80Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
| | <table><tr><td colspan='2'>[[1woj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WOJ FirstGlance]. <br> |
| |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/2',3'-cyclic-nucleotide_3'-phosphodiesterase 2',3'-cyclic-nucleotide 3'-phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.37 3.1.4.37] </span>
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| |GENE= | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1woj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1woj OCA], [https://pdbe.org/1woj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1woj RCSB], [https://www.ebi.ac.uk/pdbsum/1woj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1woj ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1woj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1woj OCA], [http://www.ebi.ac.uk/pdbsum/1woj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1woj RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/CN37_HUMAN CN37_HUMAN] May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin (By similarity). |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wo/1woj_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1woj ConSurf]. |
| | <div style="clear:both"></div> |
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| '''Crystal structure of human phosphodiesterase'''
| | ==See Also== |
| | | *[[Phosphodiesterase 3D structures|Phosphodiesterase 3D structures]] |
| | | __TOC__ |
| ==Overview==
| | </StructureSection> |
| 2',3'-Cyclic-nucleotide 3'-phosphodiesterase (CNP), a member of the 2H phosphoesterase superfamily, is firmly bound to brain white matter and found mainly in the central nervous system of vertebrates, and it catalyzes the hydrolysis of 2',3'-cyclic nucleotide to produce 2'-nucleotide. Recent studies on CNP-knockout mice have revealed that the absence of CNP causes axonal swelling and neuronal degeneration. Here, the crystal structure of the catalytic fragment (CF) of human CNP (hCNP-CF) is solved at 1.8A resolution. It is an alpha+beta type structure consisting of three alpha-helices and nine beta-strands. The structural core of the molecule is comprised of two topologically equivalent three-stranded antiparallel beta-sheets that are related by a pseudo 2-fold symmetry. Each beta-sheet contains an H-X-T-X motif, which is strictly conserved among members of the 2H phosphoesterase superfamily. The phosphate ion is bound to the side-chains of His and Thr from each of the two motifs. Structural comparison of hCNP-CF with plant 1'',2''-cyclic nucleotide phosphodiesterase (CPDase) and bacterial 2'-5' RNA ligase reveals that the H-X-T-X motifs are structurally conserved among these enzymes, but the surface properties of the active site are quite different among the enzymes, reflecting the differences in their substrates. On the basis of the present crystal structure of the hCNP-CF/phosphate complex, the available structure of the CPDase/cyclic-nucleotide analogue complex, and the recent functional studies of rat CNP-CF, we propose a possible substrate-binding mode and catalytic mechanism of CNP, which employs the nucleophilic water molecule activated by His310. The proposed mechanism is basically equivalent to the second step of the well-accepted reaction mechanism of RNase A. Since the overall structure of hCNP-CF differs considerably from that of RNase A, it is likely that the similar active sites with two catalytic histidine residues in these enzymes arose through convergent evolution.
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| ==About this Structure== | |
| 1WOJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WOJ OCA].
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| ==Reference==
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| Crystal structure of the catalytic fragment of human brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase., Sakamoto Y, Tanaka N, Ichimiya T, Kurihara T, Nakamura KT, J Mol Biol. 2005 Feb 25;346(3):789-800. Epub 2005 Jan 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713463 15713463]
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| [[Category: 2',3'-cyclic-nucleotide 3'-phosphodiesterase]]
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| [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Ichimiya, T.]] | | [[Category: Ichimiya T]] |
| [[Category: Kurihara, T.]] | | [[Category: Kurihara T]] |
| [[Category: Nakamura, K T.]] | | [[Category: Nakamura KT]] |
| [[Category: Sakamoto, Y.]] | | [[Category: Sakamoto Y]] |
| [[Category: Tanaka, N.]] | | [[Category: Tanaka N]] |
| [[Category: hydrolase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:39:11 2008''
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