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[[Image:1wn6.gif|left|200px]]


{{Structure
==Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S==
|PDB= 1wn6 |SIZE=350|CAPTION= <scene name='initialview01'>1wn6</scene>, resolution 1.80&Aring;
<StructureSection load='1wn6' size='340' side='right'caption='[[1wn6]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ARS:ARSENIC'>ARS</scene>, <scene name='pdbligand=BST:6-(4-AMINO-4-HYDROXY-2-OXO-3,4-DIHYDRO-2H-PYRIMIDIN-1-YL)-3-[3-AMINO-5-(N-METHYL-GUANIDINO)-PENT+ANOYLAMINO]-3,6-DIHYDRO-2H-PYRAN-2-CARBOXYLIC+ACID'>BST</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[1wn6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WN6 FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARS:ARSENIC'>ARS</scene>, <scene name='pdbligand=BST:6-(4-AMINO-4-HYDROXY-2-OXO-3,4-DIHYDRO-2H-PYRIMIDIN-1-YL)-3-[3-AMINO-5-(N-METHYL-GUANIDINO)-PENT+ANOYLAMINO]-3,6-DIHYDRO-2H-PYRAN-2-CARBOXYLIC+ACID'>BST</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wn6 OCA], [https://pdbe.org/1wn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wn6 RCSB], [https://www.ebi.ac.uk/pdbsum/1wn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wn6 ProSAT]</span></td></tr>
|RELATEDENTRY=[[1wn5|1WN5]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wn6 OCA], [http://www.ebi.ac.uk/pdbsum/1wn6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wn6 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/BSD_ASPTE BSD_ASPTE] Catalyzes the deamination of the cytosine moiety of the antibiotics blasticidin S, cytomycin and acetylblasticidin S.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/1wn6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wn6 ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S'''
==See Also==
 
*[[Deaminase 3D structures|Deaminase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Blasticidin S deaminase from Aspergillus terreus was crystallized with polyethylene glycol 8000. Two types of crystals were grown under the same crystallization conditions. One type grew as thin plates, while the other had a rhombic shape. The rhombic shaped crystal was suitable for high-resolution crystal structure analysis. Precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56.48 A. The calculated Vm value was acceptable when a tetramer of the enzyme was contained in an asymmetric unit. Preliminary diffraction data were collected to a resolution of 2.0 A with good statistics.
 
==About this Structure==
1WN6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WN6 OCA].
 
==Reference==
Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089374 10089374]
[[Category: Aspergillus terreus]]
[[Category: Aspergillus terreus]]
[[Category: Blasticidin-S deaminase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Furuichi M]]
[[Category: Furuichi, M.]]
[[Category: Kimura M]]
[[Category: Kimura, M.]]
[[Category: Kumasaka T]]
[[Category: Kumasaka, T.]]
[[Category: Nakasako M]]
[[Category: Nakasako, M.]]
[[Category: Ueki T]]
[[Category: Ueki, T.]]
[[Category: Yamaguchi I]]
[[Category: Yamaguchi, I.]]
[[Category: Yamamoto M]]
[[Category: Yamamoto, M.]]
[[Category: cytidine deaminase family]]
[[Category: hydrolase]]
[[Category: tetramer]]
[[Category: zinc]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:38:29 2008''

Latest revision as of 16:32, 13 March 2024

Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin SCrystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S

Structural highlights

1wn6 is a 2 chain structure with sequence from Aspergillus terreus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BSD_ASPTE Catalyzes the deamination of the cytosine moiety of the antibiotics blasticidin S, cytomycin and acetylblasticidin S.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1wn6, resolution 1.80Å

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