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==crystal structure of MHV spike protein fusion core== | |||
<StructureSection load='1wdf' size='340' side='right'caption='[[1wdf]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_A59 Murine hepatitis virus strain A59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [https://pdbe.org/1wdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [https://www.ebi.ac.uk/pdbsum/1wdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SPIKE_CVMA5 SPIKE_CVMA5] S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.<ref>PMID:16014947</ref> S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Presumably interacts with target cell lipid raft after cell attachment.<ref>PMID:16014947</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdf_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdf ConSurf]. | |||
<div style="clear:both"></div> | |||
== | ==See Also== | ||
*[[Sandbox 3001|Sandbox 3001]] | |||
*[[Spike protein 3D structures|Spike protein 3D structures]] | |||
== References == | |||
<references/> | |||
[[ | __TOC__ | ||
</StructureSection> | |||
== | [[Category: Large Structures]] | ||
< | [[Category: Murine hepatitis virus strain A59]] | ||
[[Category: Murine hepatitis virus strain | [[Category: Bai Z]] | ||
[[Category: Bai | [[Category: Gao GF]] | ||
[[Category: Gao | [[Category: Li X]] | ||
[[Category: Li | [[Category: Liu Y]] | ||
[[Category: Liu | [[Category: Lou Z]] | ||
[[Category: Lou | [[Category: Qin L]] | ||
[[Category: Qin | [[Category: Rao Z]] | ||
[[Category: Rao | [[Category: Tien P]] | ||
[[Category: Tien | [[Category: Xu Y]] | ||
[[Category: Xu | |||
Latest revision as of 16:31, 13 March 2024
crystal structure of MHV spike protein fusion corecrystal structure of MHV spike protein fusion core
Structural highlights
FunctionSPIKE_CVMA5 S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.[1] S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Presumably interacts with target cell lipid raft after cell attachment.[2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. See AlsoReferences
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