1rwt: Difference between revisions

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-[[Image:1rwt.gif|left|200px]]<br />
-<applet load="1rwt" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1rwt, resolution 2.72&Aring;" />
-'''Crystal Structure of Spinach Major Light-harvesting complex at 2.72 Angstrom Resolution'''<br />
-==Overview==
+==Crystal Structure of Spinach Major Light-harvesting complex at 2.72 Angstrom Resolution==
-The major light-harvesting complex of photosystem II (LHC-II) serves as, the principal solar energy collector in the photosynthesis of green plants, and presumably also functions in photoprotection under high-light, conditions. Here we report the first X-ray structure of LHC-II in, icosahedral proteoliposome assembly at atomic detail. One asymmetric unit, of a large R32 unit cell contains ten LHC-II monomers. The 14 chlorophylls, (Chl) in each monomer can be unambiguously distinguished as eight Chla and, six Chlb molecules. Assignment of the orientation of the transition dipole, moment of each chlorophyll has been achieved. All Chlb are located around, the interface between adjacent monomers, and together with Chla they are, the basis for efficient light harvesting. Four carotenoid-binding sites, per monomer have been observed. The xanthophyll-cycle carotenoid at the, monomer-monomer interface may be involved in the non-radiative dissipation, of excessive energy, one of the photoprotective strategies that have, evolved in plants.
+<StructureSection load='1rwt' size='340' side='right'caption='[[1rwt]], [[Resolution|resolution]] 2.72&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1rwt]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. The November 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Photosystem II''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_11 10.2210/rcsb_pdb/mom_2004_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RWT FirstGlance]. <br>
-RWT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with BNG, NA, LUT, XAT, NEX, LHG, DGD, CHL and CLA as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1RWT with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb59_1.html Photosystem II]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RWT OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.72&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=CHL:CHLOROPHYLL+B'>CHL</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=LUT:(3R,3R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3-DIOL'>LUT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NEX:(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL'>NEX</scene>, <scene name='pdbligand=XAT:(3S,5R,6S,3S,5R,6S)-5,6,5,6-DIEPOXY-5,6,5,6-+TETRAHYDRO-BETA,BETA-CAROTENE-3,3-DIOL'>XAT</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwt OCA], [https://pdbe.org/1rwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rwt RCSB], [https://www.ebi.ac.uk/pdbsum/1rwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rwt ProSAT]</span></td></tr>
-Crystal structure of spinach major light-harvesting complex at 2.72 A resolution., Liu Z, Yan H, Wang K, Kuang T, Zhang J, Gui L, An X, Chang W, Nature. 2004 Mar 18;428(6980):287-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15029188 15029188]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CB2A_SPIOL CB2A_SPIOL] The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rw/1rwt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rwt ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Photosystem II]]
-[[Category: Single protein]]
+[[Category: RCSB PDB Molecule of the Month]]
 [[Category: Spinacia oleracea]]
-[[Category: An, X.]]
+[[Category: An X]]
-[[Category: Chang, W.]]
+[[Category: Chang W]]
-[[Category: Gui, L.]]
+[[Category: Gui L]]
-[[Category: Kuang, T.]]
+[[Category: Kuang T]]
-[[Category: Liu, Z.]]
+[[Category: Liu Z]]
-[[Category: Wang, K.]]
+[[Category: Wang K]]
-[[Category: Yan, H.]]
+[[Category: Yan H]]
-[[Category: Zhang, J.]]
+[[Category: Zhang J]]
-[[Category: BNG]]
-[[Category: CHL]]
-[[Category: CLA]]
-[[Category: DGD]]
-[[Category: LHG]]
-[[Category: LUT]]
-[[Category: NA]]
-[[Category: NEX]]
-[[Category: XAT]]
-[[Category: light-harvesting complex]]
-[[Category: membrane protein]]
-[[Category: plant]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:05:48 2007''