1our: Difference between revisions

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New page: left|200px<br /><applet load="1our" size="450" color="white" frame="true" align="right" spinBox="true" caption="1our, resolution 1.42Å" /> '''LecB (PA-LII) in com...
 
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[[Image:1our.jpg|left|200px]]<br /><applet load="1our" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1our, resolution 1.42&Aring;" />
'''LecB (PA-LII) in complex with mannose'''<br />


==Overview==
==LecB (PA-LII) in complex with mannose==
The crystal structure of Pseudomonas aeruginosa fucose-specific lectin, LecB was determined in its metal-bound and metal-free state as well as in, complex with fucose, mannose and fructopyranose. All three monosaccharides, bind isosterically via direct interactions with two calcium ions as well, as direct hydrogen bonds with several side-chains. The higher affinity for, fucose is explained by the details of the binding site around C6 and O1 of, fucose. In the mannose and fructose complexes, a carboxylate oxygen atom, and one or two hydroxyl groups are partly shielded from solvent upon sugar, binding, preventing them from completely fulfilling their hydrogen bonding, potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon, demetallization, the C terminus as well as the otherwise rigid, metal-binding loop become more mobile and adopt multiple conformations.
<StructureSection load='1our' size='340' side='right'caption='[[1our]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1our]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OUR FirstGlance]. <br>
1OUR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with MAN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OUR OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.42&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1our FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1our OCA], [https://pdbe.org/1our PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1our RCSB], [https://www.ebi.ac.uk/pdbsum/1our PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1our ProSAT]</span></td></tr>
Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12909014 12909014]
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HYN5_PSEAE Q9HYN5_PSEAE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ou/1our_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1our ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Jaeger K-E]]
[[Category: Jaeger, K.E.]]
[[Category: Loris R]]
[[Category: Loris, R.]]
[[Category: Tielker D]]
[[Category: Tielker, D.]]
[[Category: Wyns L]]
[[Category: Wyns, L.]]
[[Category: CA]]
[[Category: MAN]]
[[Category: carbohydrate]]
[[Category: lectin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:27:54 2007''

Latest revision as of 16:28, 13 March 2024

LecB (PA-LII) in complex with mannoseLecB (PA-LII) in complex with mannose

Structural highlights

1our is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.42Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9HYN5_PSEAE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1our, resolution 1.42Å

Drag the structure with the mouse to rotate

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OCA
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