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[[Image:1n3o.gif|left|200px]]<br /><applet load="1n3o" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1n3o, resolution 2.0&Aring;" />
'''Pterocarcpus angolensis lectin in complex with alpha-methyl glucose'''<br />


==Overview==
==Pterocarcpus angolensis lectin in complex with alpha-methyl glucose==
The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site.
<StructureSection load='1n3o' size='340' side='right'caption='[[1n3o]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1n3o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3O FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GYP:METHYL-ALPHA-D-GLUCOPYRANOSIDE'>GYP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3o OCA], [https://pdbe.org/1n3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3o RCSB], [https://www.ebi.ac.uk/pdbsum/1n3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3o ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8GSD2_PTEAG Q8GSD2_PTEAG]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3o_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3o ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1N3O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis] with <scene name='pdbligand=GYP:'>GYP</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3O OCA].
*[[Pal|Pal]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose., Loris R, Imberty A, Beeckmans S, Van Driessche E, Read JS, Bouckaert J, De Greve H, Buts L, Wyns L, J Biol Chem. 2003 May 2;278(18):16297-303. Epub 2003 Feb 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12595543 12595543]
[[Category: Large Structures]]
[[Category: Pterocarpus angolensis]]
[[Category: Pterocarpus angolensis]]
[[Category: Single protein]]
[[Category: Beeckmans S]]
[[Category: Beeckmans, S.]]
[[Category: Bouckaert J]]
[[Category: Bouckaert, J.]]
[[Category: Buts L]]
[[Category: Buts, L.]]
[[Category: De Greve H]]
[[Category: Driessche, E Van.]]
[[Category: Imberty A]]
[[Category: Greve, H De.]]
[[Category: Loris R]]
[[Category: Imberty, A.]]
[[Category: Read JS]]
[[Category: Loris, R.]]
[[Category: Van Driessche E]]
[[Category: Read, J S.]]
[[Category: Wyns L]]
[[Category: Wyns, L.]]
[[Category: CA]]
[[Category: GYP]]
[[Category: MN]]
[[Category: carbohydrate recognition]]
[[Category: glucose]]
[[Category: lectin]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:52 2008''

Latest revision as of 16:27, 13 March 2024

Pterocarcpus angolensis lectin in complex with alpha-methyl glucosePterocarcpus angolensis lectin in complex with alpha-methyl glucose

Structural highlights

1n3o is a 2 chain structure with sequence from Pterocarpus angolensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8GSD2_PTEAG

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1n3o, resolution 2.00Å

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