1mp9: Difference between revisions

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==TBP from a mesothermophilic archaeon, Sulfolobus acidocaldarius==
==TBP from a mesothermophilic archaeon, Sulfolobus acidocaldarius==
<StructureSection load='1mp9' size='340' side='right' caption='[[1mp9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='1mp9' size='340' side='right'caption='[[1mp9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1mp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MP9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1mp9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MP9 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mp9 OCA], [http://pdbe.org/1mp9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mp9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mp9 PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mp9 OCA], [https://pdbe.org/1mp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mp9 RCSB], [https://www.ebi.ac.uk/pdbsum/1mp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mp9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TBP_SULAC TBP_SULAC]] General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation (By similarity).  
[https://www.uniprot.org/uniprot/TBP_SULAC TBP_SULAC] General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mp9_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mp9_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mp9 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of TATA binding protein (TBP) from a mesothermophilic archaeon, Sulfolobus acidocaldarius, has been determined at a resolution of 2.0 A with an R factor of 20.9%. By comparing this structure with the structures of TBPs from a hyperthermophilic archaeon and mesophilic eukaryotes, as well as by comparing amino acid sequences of TBPs from archaea, covering a wide range of optimum growth temperatures, two significant determinants of the stability of TBP have been identified: increasing the interior hydrophobicity by interaction between three residues, Val, Leu, and Ile, with further differentiation of the surface, and increasing its hydrophilicity and raising the cost of unfolding. These findings suggest directions along which the stability of TBP can be engineered.
Origins of protein stability revealed by comparing crystal structures of TATA binding proteins.,Koike H, Kawashima-Ohya Y, Yamasaki T, Clowney L, Katsuya Y, Suzuki M Structure. 2004 Jan;12(1):157-68. PMID:14725775<ref>PMID:14725775</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1mp9" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[TATA-Binding Protein|TATA-Binding Protein]]
*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 33909]]
[[Category: Large Structures]]
[[Category: Clowney, L]]
[[Category: Sulfolobus acidocaldarius]]
[[Category: Katsuya, Y]]
[[Category: Clowney L]]
[[Category: Kawashima-Ohya, Y]]
[[Category: Katsuya Y]]
[[Category: Koike, H]]
[[Category: Kawashima-Ohya Y]]
[[Category: Suzuki, M]]
[[Category: Koike H]]
[[Category: Yamasaki, T]]
[[Category: Suzuki M]]
[[Category: Dna binding protein]]
[[Category: Yamasaki T]]
[[Category: Dna-binding protein]]
[[Category: Transcription factor]]
[[Category: Transcription regulation]]

Latest revision as of 16:27, 13 March 2024

TBP from a mesothermophilic archaeon, Sulfolobus acidocaldariusTBP from a mesothermophilic archaeon, Sulfolobus acidocaldarius

Structural highlights

1mp9 is a 2 chain structure with sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TBP_SULAC General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1mp9, resolution 2.00Å

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