1mp9: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1mp9.gif|left|200px]]<br /><applet load="1mp9" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1mp9, resolution 2.000&Aring;" />
-'''TBP from a mesothermophilic archaeon, Sulfolobus acidocaldarius'''<br />
-==Overview==
+==TBP from a mesothermophilic archaeon, Sulfolobus acidocaldarius==
-The crystal structure of TATA binding protein (TBP) from a mesothermophilic archaeon, Sulfolobus acidocaldarius, has been determined at a resolution of 2.0 A with an R factor of 20.9%. By comparing this structure with the structures of TBPs from a hyperthermophilic archaeon and mesophilic eukaryotes, as well as by comparing amino acid sequences of TBPs from archaea, covering a wide range of optimum growth temperatures, two significant determinants of the stability of TBP have been identified: increasing the interior hydrophobicity by interaction between three residues, Val, Leu, and Ile, with further differentiation of the surface, and increasing its hydrophilicity and raising the cost of unfolding. These findings suggest directions along which the stability of TBP can be engineered.
+<StructureSection load='1mp9' size='340' side='right'caption='[[1mp9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1mp9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MP9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mp9 OCA], [https://pdbe.org/1mp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mp9 RCSB], [https://www.ebi.ac.uk/pdbsum/1mp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mp9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TBP_SULAC TBP_SULAC] General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mp9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mp9 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-MP9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP9 OCA].
+*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Origins of protein stability revealed by comparing crystal structures of TATA binding proteins., Koike H, Kawashima-Ohya Y, Yamasaki T, Clowney L, Katsuya Y, Suzuki M, Structure. 2004 Jan;12(1):157-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14725775 14725775]
+[[Category: Large Structures]]
-[[Category: Single protein]]
 [[Category: Sulfolobus acidocaldarius]]
-[[Category: Clowney, L.]]
+[[Category: Clowney L]]
-[[Category: Katsuya, Y.]]
+[[Category: Katsuya Y]]
-[[Category: Kawashima-Ohya, Y.]]
+[[Category: Kawashima-Ohya Y]]
-[[Category: Koike, H.]]
+[[Category: Koike H]]
-[[Category: Suzuki, M.]]
+[[Category: Suzuki M]]
-[[Category: Yamasaki, T.]]
+[[Category: Yamasaki T]]
-[[Category: dna-binding protein]]
-[[Category: transcription factor]]
-[[Category: transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:39 2008''