|
|
| (One intermediate revision by the same user not shown) |
| Line 1: |
Line 1: |
|
| |
|
| ==Oxidoreductase IruO in the oxidized form== | | ==Oxidoreductase IruO in the oxidized form== |
| <StructureSection load='5twc' size='340' side='right' caption='[[5twc]], [[Resolution|resolution]] 2.31Å' scene=''> | | <StructureSection load='5twc' size='340' side='right'caption='[[5twc]], [[Resolution|resolution]] 2.31Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5twc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TWC FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5twc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_USA300 Staphylococcus aureus subsp. aureus USA300]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TWC FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5twb|5twb]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5twc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5twc OCA], [https://pdbe.org/5twc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5twc RCSB], [https://www.ebi.ac.uk/pdbsum/5twc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5twc ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5twc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5twc OCA], [http://pdbe.org/5twc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5twc RCSB], [http://www.ebi.ac.uk/pdbsum/5twc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5twc ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/FENR_STAA3 FENR_STAA3] |
| Many pathogenic bacteria including Staphylococcus aureus use iron-chelating siderophores to acquire iron. Iron uptake oxidoreductase (IruO), a flavin adenine dinucleotide (FAD)-containing nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reductase from S. aureus, functions as a reductase for IsdG and IsdI, two paralogous heme degrading enzymes. Also, the gene encoding for IruO was shown to be required for growth of S. aureus on hydroxamate siderophores as a sole iron source. Here, we show that IruO binds the hydroxamate-type siderophores desferrioxamine B and ferrichrome A with low micromolar affinity and in the presence of NADPH, Fe(II) was released. Steady-state kinetics of Fe(II) release provides kcat/Km values in the range of 600 to 7000 M-1 s-1 for these siderophores supporting a role for IruO as a siderophore reductase in iron utilization. Crystal structures of IruO were solved in two distinct conformational states mediated by the formation of an intramolecular disulfide bond. A putative siderophore binding site was identified adjacent to the FAD cofactor. This site is partly occluded in the oxidized IruO structure consistent with this form being less active than reduced IruO. This reduction in activity could have a physiological role to limit iron release under oxidative stress conditions. Visible spectroscopy of anaerobically reduced IruO showed that the reaction proceeds by a single electron transfer mechanism through an FAD semiquinone intermediate. From the data, a model for single electron siderophore reduction by IruO using NADPH is described.
| |
| | |
| Iron Uptake Oxidoreductase (IruO) Uses a Flavin Adenine Dinucleotide Semiquinone Intermediate for Iron-Siderophore Reduction.,Kobylarz MJ, Heieis GA, Loutet SA, Murphy MEP ACS Chem Biol. 2017 Jul 21;12(7):1778-1786. doi: 10.1021/acschembio.7b00203. Epub, 2017 May 12. PMID:28463500<ref>PMID:28463500</ref>
| |
| | |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 5twc" style="background-color:#fffaf0;"></div>
| |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Heieis, G A]] | | [[Category: Large Structures]] |
| [[Category: Kobylarz, M J]]
| | [[Category: Staphylococcus aureus subsp. aureus USA300]] |
| [[Category: Loutet, S A]]
| | [[Category: Heieis GA]] |
| [[Category: Murphy, M E.P]] | | [[Category: Kobylarz MJ]] |
| [[Category: Flavin adenine dinucleotide]] | | [[Category: Loutet SA]] |
| [[Category: Iron]] | | [[Category: Murphy MEP]] |
| [[Category: Oxidoreductase]] | |
| [[Category: Siderophore]] | |