6vym: Difference between revisions
New page: '''Unreleased structure''' The entry 6vym is ON HOLD Authors: Description: Category: Unreleased Structures |
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The | ==Cryo-EM structure of mechanosensitive channel MscS in PC-18:1 nanodiscs treated with beta-cyclodextran== | ||
<StructureSection load='6vym' size='340' side='right'caption='[[6vym]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6vym]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VYM FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vym OCA], [https://pdbe.org/6vym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vym RCSB], [https://www.ebi.ac.uk/pdbsum/6vym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vym ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/MSCS_ECOLI MSCS_ECOLI] Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell, opening in response to stretch forces in the membrane lipid bilayer, without the need for other proteins. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage; as the membrane is depolarized, less tension is required to open the channel and vice versa. The channel is characterized by short bursts of activity that last for a few seconds. The channel pore is formed by TM3 and the loop between TM2 and TM3. After a sharp turn at Gly-113, an alpha-helix (residues 114-127) is oriented nearly parallel to the plane of the putative lipid bilayer. On the intracellular side of the channel, the permeation pathway of MscS does not connect directly to the cytoplasm but instead opens to a large chamber that is connected to the cytoplasm. This chamber resembles a molecular filter that could serve to prescreen large molecules before they are allowed passage to the transmembrane pore. The TM1 and TM2 helices appear to be likely candidates for mediating the tension and voltage sensitivities of MscS. Gating requires large rearrangements of at least the C-terminus. | |||
==See Also== | |||
*[[Ion channels 3D structures|Ion channels 3D structures]] | |||
__TOC__ | |||
[[Category: | </StructureSection> | ||
[[Category: Escherichia coli]] | |||
[[Category: Large Structures]] | |||
[[Category: Cox CD]] | |||
[[Category: Daday C]] | |||
[[Category: Groot B]] | |||
[[Category: Gu R]] | |||
[[Category: Martinac B]] | |||
[[Category: Walz T]] | |||
[[Category: Zhang Y]] | |||