6vws: Difference between revisions

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<StructureSection load='6vws' size='340' side='right'caption='[[6vws]], [[Resolution|resolution]] 6.08&Aring;' scene=''>
<StructureSection load='6vws' size='340' side='right'caption='[[6vws]], [[Resolution|resolution]] 6.08&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6vws]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VWS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VWS FirstGlance]. <br>
<table><tr><td colspan='2'>[[6vws]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VWS FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.08&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vws OCA], [http://pdbe.org/6vws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vws RCSB], [http://www.ebi.ac.uk/pdbsum/6vws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vws ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vws OCA], [https://pdbe.org/6vws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vws RCSB], [https://www.ebi.ac.uk/pdbsum/6vws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vws ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/GAG_HV1N5 GAG_HV1N5] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu.  Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers.  p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).
The potent HIV-1 capsid inhibitor GS-6207 is an investigational principal component of long-acting antiretroviral therapy. We found that GS-6207 inhibits HIV-1 by stabilizing and thereby preventing functional disassembly of the capsid shell in infected cells. X-ray crystallography, cryo-electron microscopy, and hydrogen-deuterium exchange experiments revealed that GS-6207 tightly binds two adjoining capsid subunits and promotes distal intra- and inter-hexamer interactions that stabilize the curved capsid lattice. In addition, GS-6207 interferes with capsid binding to the cellular HIV-1 cofactors Nup153 and CPSF6 that mediate viral nuclear import and direct integration into gene-rich regions of chromatin. These findings elucidate structural insights into the multimodal, potent antiviral activity of GS-6207 and provide a means for rationally developing second-generation therapies.


Structural and mechanistic bases for a potent HIV-1 capsid inhibitor.,Bester SM, Wei G, Zhao H, Adu-Ampratwum D, Iqbal N, Courouble VV, Francis AC, Annamalai AS, Singh PK, Shkriabai N, Van Blerkom P, Morrison J, Poeschla EM, Engelman AN, Melikyan GB, Griffin PR, Fuchs JR, Asturias FJ, Kvaratskhelia M Science. 2020 Oct 16;370(6514):360-364. doi: 10.1126/science.abb4808. PMID:33060363<ref>PMID:33060363</ref>
==See Also==
 
*[[Gag polyprotein 3D structures|Gag polyprotein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
</div>
<div class="pdbe-citations 6vws" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human immunodeficiency virus 1]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Asturias, F]]
[[Category: Asturias F]]
[[Category: Iqbal, N]]
[[Category: Iqbal N]]
[[Category: Kvaratskhelia, M]]
[[Category: Kvaratskhelia M]]
[[Category: Vanblerkom, P]]
[[Category: Vanblerkom P]]
[[Category: Zhao, H]]
[[Category: Zhao H]]
[[Category: Gs-6207 hexamer hiv]]
[[Category: Helical reconstruction]]
[[Category: Rastr]]
[[Category: Viral protein]]

Latest revision as of 17:40, 6 March 2024

Hexamer of Helical HIV capsid by RASTR methodHexamer of Helical HIV capsid by RASTR method

Structural highlights

6vws is a 6 chain structure with sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 6.08Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GAG_HV1N5 Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).

See Also

6vws, resolution 6.08Å

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