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==Crystal structure of FePYR1 in complex with abscisic acid==
==Crystal structure of FePYR1 in complex with abscisic acid==
<StructureSection load='5ujv' size='340' side='right' caption='[[5ujv]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='5ujv' size='340' side='right'caption='[[5ujv]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5ujv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Festuca_elata Festuca elata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UJV FirstGlance]. <br>
<table><tr><td colspan='2'>[[5ujv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Festuca_elata Festuca elata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UJV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ujv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ujv OCA], [http://pdbe.org/5ujv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ujv RCSB], [http://www.ebi.ac.uk/pdbsum/5ujv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ujv ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ujv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ujv OCA], [https://pdbe.org/5ujv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ujv RCSB], [https://www.ebi.ac.uk/pdbsum/5ujv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ujv ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A2D1UXN2_9POAL A0A2D1UXN2_9POAL]
Turfgrass are widely cultivated ornamental plants that have important ecological, societal and economical values. However, many turfgrass species are susceptible to drought and demand frequent irrigation thus consuming large amounts of water. With the ultimate goal of improving drought resistance in turfgrass, we identified several ABA receptors in turfgrass that are important to mediate ABA signaling and drought stress response. The ABA receptor FePYR1 from turfgrass Festuca elata was demonstrated to bind ABA as a monomer. Crystal structure analysis revealed that FePYR1 recognizes and binds ABA by the common gate-latch-lock mechanism resembling the Arabidopsis ABA receptors, but the ABA binding pocket in FePYR1 shows discrepant residues resulting in different binding affinity to ABA. Structure-guided alterations of amino acid residues in FePYR1 generated ABA receptor variants with significantly increased ABA binding affinity. Expression of FePYR1 in Arabidopsis conferred enhanced drought resistance in the transgenic plants. These findings provided detailed information about FePYR1 and demonstrated that structure-assisted engineering could create superior ABA receptors for improving plant drought resistance. The detailed structural information of FePYR1 would also assist future rational design of small molecules targeting specific ABA receptors in economically important plant species.
 
Structure determination and activity manipulation of the turfgrass ABA receptor FePYR1.,Ren Z, Wang Z, Zhou XE, Shi H, Hong Y, Cao M, Chan Z, Liu X, Xu HE, Zhu JK Sci Rep. 2017 Oct 25;7(1):14022. doi: 10.1038/s41598-017-14101-9. PMID:29070857<ref>PMID:29070857</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ujv" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Abscisic acid receptor|Abscisic acid receptor]]
*[[Abscisic acid receptor 3D structures|Abscisic acid receptor 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Festuca elata]]
[[Category: Festuca elata]]
[[Category: Cao, M]]
[[Category: Large Structures]]
[[Category: Chan, Z]]
[[Category: Cao M]]
[[Category: Hong, Y]]
[[Category: Chan Z]]
[[Category: Liu, X]]
[[Category: Hong Y]]
[[Category: Ren, Z]]
[[Category: Liu X]]
[[Category: Shi, H]]
[[Category: Ren Z]]
[[Category: Wang, Z]]
[[Category: Shi H]]
[[Category: Xu, H E]]
[[Category: Wang Z]]
[[Category: Zhou, X E]]
[[Category: Xu HE]]
[[Category: Zhu, J K]]
[[Category: Zhou XE]]
[[Category: Abscisic acid binding protein]]
[[Category: Zhu J-K]]
[[Category: Abscisic acid receptor]]
[[Category: Drought resistance]]
[[Category: Turfgrass]]

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