5l2x: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 5l2x is ON HOLD  until Paper Publication
==Crystal structure of human PrimPol ternary complex==
 
<StructureSection load='5l2x' size='340' side='right'caption='[[5l2x]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
Authors: Rechkoblit, O., Gupta, Y.K., Malik, R., Rajashankar, K.R., Johnson, R.E., Prakash, L., Prakash, S., Aggarwal, A.K.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[5l2x]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L2X FirstGlance]. <br>
Description: Crystal structure of human PrimPol ternary complex
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
[[Category: Unreleased Structures]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene>, <scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene></td></tr>
[[Category: Rajashankar, K.R]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l2x OCA], [https://pdbe.org/5l2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l2x RCSB], [https://www.ebi.ac.uk/pdbsum/5l2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l2x ProSAT]</span></td></tr>
[[Category: Malik, R]]
</table>
[[Category: Johnson, R.E]]
== Disease ==
[[Category: Rechkoblit, O]]
[https://www.uniprot.org/uniprot/PRIPO_HUMAN PRIPO_HUMAN] The disease is caused by mutations affecting the gene represented in this entry.
[[Category: Prakash, S]]
== Function ==
[[Category: Prakash, L]]
[https://www.uniprot.org/uniprot/PRIPO_HUMAN PRIPO_HUMAN] DNA primase and DNA polymerase able to initiate de novo DNA synthesis using dNTPs. Shows a high capacity to tolerate DNA damage lesions such as 8oxoG and abasic sites in DNA. Involved in translesion synthesis via its primase activity by mediating uninterrupted fork progression after programmed or damage-induced fork arrest and by reinitiating DNA synthesis after dNTP depletion. Required for mitochondrial DNA (mtDNA) synthesis, suggesting it may be involved in DNA tolerance during the replication of mitochondrial DNA. Has non-overlapping function with POLH.<ref>PMID:24126761</ref> <ref>PMID:24207056</ref> <ref>PMID:24240614</ref> <ref>PMID:24267451</ref>
[[Category: Gupta, Y.K]]
== References ==
[[Category: Aggarwal, A.K]]
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Aggarwal AK]]
[[Category: Gupta YK]]
[[Category: Johnson RE]]
[[Category: Malik R]]
[[Category: Prakash L]]
[[Category: Prakash S]]
[[Category: Rajashankar KR]]
[[Category: Rechkoblit O]]

Latest revision as of 15:43, 6 March 2024

Crystal structure of human PrimPol ternary complexCrystal structure of human PrimPol ternary complex

Structural highlights

5l2x is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

PRIPO_HUMAN The disease is caused by mutations affecting the gene represented in this entry.

Function

PRIPO_HUMAN DNA primase and DNA polymerase able to initiate de novo DNA synthesis using dNTPs. Shows a high capacity to tolerate DNA damage lesions such as 8oxoG and abasic sites in DNA. Involved in translesion synthesis via its primase activity by mediating uninterrupted fork progression after programmed or damage-induced fork arrest and by reinitiating DNA synthesis after dNTP depletion. Required for mitochondrial DNA (mtDNA) synthesis, suggesting it may be involved in DNA tolerance during the replication of mitochondrial DNA. Has non-overlapping function with POLH.[1] [2] [3] [4]

References

  1. ↑ Wan L, Lou J, Xia Y, Su B, Liu T, Cui J, Sun Y, Lou H, Huang J. hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity. EMBO Rep. 2013 Dec;14(12):1104-12. doi: 10.1038/embor.2013.159. Epub 2013 Oct 15. PMID:24126761 doi:http://dx.doi.org/10.1038/embor.2013.159
  2. ↑ Garcia-Gomez S, Reyes A, Martinez-Jimenez MI, Chocron ES, Mouron S, Terrados G, Powell C, Salido E, Mendez J, Holt IJ, Blanco L. PrimPol, an archaic primase/polymerase operating in human cells. Mol Cell. 2013 Nov 21;52(4):541-53. doi: 10.1016/j.molcel.2013.09.025. Epub 2013 , Oct 24. PMID:24207056 doi:http://dx.doi.org/10.1016/j.molcel.2013.09.025
  3. ↑ Mouron S, Rodriguez-Acebes S, Martinez-Jimenez MI, Garcia-Gomez S, Chocron S, Blanco L, Mendez J. Repriming of DNA synthesis at stalled replication forks by human PrimPol. Nat Struct Mol Biol. 2013 Dec;20(12):1383-9. doi: 10.1038/nsmb.2719. Epub 2013, Nov 17. PMID:24240614 doi:http://dx.doi.org/10.1038/nsmb.2719
  4. ↑ Bianchi J, Rudd SG, Jozwiakowski SK, Bailey LJ, Soura V, Taylor E, Stevanovic I, Green AJ, Stracker TH, Lindsay HD, Doherty AJ. PrimPol bypasses UV photoproducts during eukaryotic chromosomal DNA replication. Mol Cell. 2013 Nov 21;52(4):566-73. doi: 10.1016/j.molcel.2013.10.035. PMID:24267451 doi:http://dx.doi.org/10.1016/j.molcel.2013.10.035

5l2x, resolution 2.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=5l2x&oldid=4081106"