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==Structure of a 5-hydroxytryptophan aptamer==
==Structure of a 5-hydroxytryptophan aptamer==
<StructureSection load='5kpy' size='340' side='right' caption='[[5kpy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='5kpy' size='340' side='right'caption='[[5kpy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5kpy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KPY FirstGlance]. <br>
<table><tr><td colspan='2'>[[5kpy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KPY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4PQ:5-HYDROXY-L-TRYPTOPHAN'>4PQ</scene>, <scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kpy OCA], [http://pdbe.org/5kpy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kpy RCSB], [http://www.ebi.ac.uk/pdbsum/5kpy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kpy ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4PQ:5-HYDROXY-L-TRYPTOPHAN'>4PQ</scene>, <scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kpy OCA], [https://pdbe.org/5kpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kpy RCSB], [https://www.ebi.ac.uk/pdbsum/5kpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kpy ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Allosteric RNA devices are increasingly being viewed as important tools capable of monitoring enzyme evolution, optimizing engineered metabolic pathways, facilitating gene discovery and regulators of nucleic acid-based therapeutics. A key bottleneck in the development of these platforms is the availability of small-molecule-binding RNA aptamers that robustly function in the cellular environment. Although aptamers can be raised against nearly any desired target through in vitro selection, many cannot easily be integrated into devices or do not reliably function in a cellular context. Here, we describe a new approach using secondary- and tertiary-structural scaffolds derived from biologically active riboswitches and small ribozymes. When applied to the neurotransmitter precursors 5-hydroxytryptophan and 3,4-dihydroxyphenylalanine, this approach yielded easily identifiable and characterizable aptamers predisposed for coupling to readout domains to allow engineering of nucleic acid-sensory devices that function in vitro and in the cellular context.
Recurrent RNA motifs as scaffolds for genetically encodable small-molecule biosensors.,Porter EB, Polaski JT, Morck MM, Batey RT Nat Chem Biol. 2017 Jan 16. doi: 10.1038/nchembio.2278. PMID:28092358<ref>PMID:28092358</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5kpy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Batey, R T]]
[[Category: Large Structures]]
[[Category: Merck, M]]
[[Category: Unidentified]]
[[Category: Porter, E]]
[[Category: Batey RT]]
[[Category: 5-hydroxytryptophan]]
[[Category: Merck M]]
[[Category: Aptamer]]
[[Category: Porter E]]
[[Category: Riboswitch]]
[[Category: Rna]]

Latest revision as of 15:41, 6 March 2024

Structure of a 5-hydroxytryptophan aptamerStructure of a 5-hydroxytryptophan aptamer

Structural highlights

5kpy is a 1 chain structure with sequence from Unidentified. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

5kpy, resolution 2.00Å

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