|
|
| (2 intermediate revisions by the same user not shown) |
| Line 3: |
Line 3: |
| <SX load='5k0z' size='340' side='right' viewer='molstar' caption='[[5k0z]], [[Resolution|resolution]] 2.80Å' scene=''> | | <SX load='5k0z' size='340' side='right' viewer='molstar' caption='[[5k0z]], [[Resolution|resolution]] 2.80Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5k0z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K0Z FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5k0z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K0Z FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k10|5k10]], [[5k11|5k11]], [[5k12|5k12]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LDHB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k0z OCA], [https://pdbe.org/5k0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k0z RCSB], [https://www.ebi.ac.uk/pdbsum/5k0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k0z ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase L-lactate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.27 1.1.1.27] </span></td></tr>
| |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k0z OCA], [http://pdbe.org/5k0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k0z RCSB], [http://www.ebi.ac.uk/pdbsum/5k0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k0z ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/LDHB_CHICK LDHB_CHICK] |
| Recent advances in single-particle cryoelecton microscopy (cryo-EM) are enabling generation of numerous near-atomic resolution structures for well-ordered protein complexes with sizes >/= approximately 200 kDa. Whether cryo-EM methods are equally useful for high-resolution structural analysis of smaller, dynamic protein complexes such as those involved in cellular metabolism remains an important question. Here, we present 3.8 A resolution cryo-EM structures of the cancer target isocitrate dehydrogenase (93 kDa) and identify the nature of conformational changes induced by binding of the allosteric small-molecule inhibitor ML309. We also report 2.8-A- and 1.8-A-resolution structures of lactate dehydrogenase (145 kDa) and glutamate dehydrogenase (334 kDa), respectively. With these results, two perceived barriers in single-particle cryo-EM are overcome: (1) crossing 2 A resolution and (2) obtaining structures of proteins with sizes < 100 kDa, demonstrating that cryo-EM can be used to investigate a broad spectrum of drug-target interactions and dynamic conformational states.
| |
| | |
| Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery.,Merk A, Bartesaghi A, Banerjee S, Falconieri V, Rao P, Davis MI, Pragani R, Boxer MB, Earl LA, Milne JL, Subramaniam S Cell. 2016 Jun 16;165(7):1698-707. doi: 10.1016/j.cell.2016.05.040. Epub 2016 May, 26. PMID:27238019<ref>PMID:27238019</ref>
| |
| | |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 5k0z" style="background-color:#fffaf0;"></div>
| |
|
| |
|
| ==See Also== | | ==See Also== |
| *[[Lactate dehydrogenase 3D structures|Lactate dehydrogenase 3D structures]] | | *[[Lactate dehydrogenase 3D structures|Lactate dehydrogenase 3D structures]] |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Chick]] | | [[Category: Gallus gallus]] |
| [[Category: L-lactate dehydrogenase]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Banerjee, S]] | | [[Category: Banerjee S]] |
| [[Category: Bartesaghi, A]] | | [[Category: Bartesaghi A]] |
| [[Category: Earl, L]] | | [[Category: Earl L]] |
| [[Category: Falconieri, V]] | | [[Category: Falconieri V]] |
| [[Category: Merk, A]] | | [[Category: Merk A]] |
| [[Category: Milne, J]] | | [[Category: Milne J]] |
| [[Category: Rao, P]] | | [[Category: Rao P]] |
| [[Category: Subramaniam, S]] | | [[Category: Subramaniam S]] |
| [[Category: Lactate dehydrogenase]]
| |
| [[Category: Oxidoreductase]]
| |
| [[Category: Small metabolic complex]]
| |
| [[Category: Small molecule inhibitor]]
| |