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==Crystal structure of Chaetomium thermophilum Nup192==
==Crystal structure of Chaetomium thermophilum Nup192==
<StructureSection load='5hb4' size='340' side='right' caption='[[5hb4]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='5hb4' size='340' side='right'caption='[[5hb4]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5hb4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HB4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5hb4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HB4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OS:OSMIUM+ION'>OS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hax|5hax]], [[5hay|5hay]], [[5haz|5haz]], [[5hb2|5hb2]], [[5hb1|5hb1]], [[5hb0|5hb0]], [[5hb3|5hb3]], [[5hb5|5hb5]], [[5hb6|5hb6]], [[5hb7|5hb7]], [[5hb8|5hb8]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OS:OSMIUM+ION'>OS</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NUP192, CTHT_0023410 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hb4 OCA], [https://pdbe.org/5hb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hb4 RCSB], [https://www.ebi.ac.uk/pdbsum/5hb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hb4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hb4 OCA], [http://pdbe.org/5hb4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hb4 RCSB], [http://www.ebi.ac.uk/pdbsum/5hb4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hb4 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/NU192_CHATD NU192_CHATD] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP192 is located to the NPC core at the nuclear membrane and is essential for de novo assembly of NPCs.[UniProtKB:P47054]
The nuclear pore complex (NPC) controls the transport of macromolecules between the nucleus and cytoplasm, but its molecular architecture has thus far remained poorly defined. We biochemically reconstituted NPC core protomers and elucidated the underlying protein-protein interaction network. Flexible linker sequences, rather than interactions between the structured core scaffold nucleoporins, mediate the assembly of the inner ring complex and its attachment to the NPC coat. X-ray crystallographic analysis of these scaffold nucleoporins revealed the molecular details of their interactions with the flexible linker sequences and enabled construction of full-length atomic structures. By docking these structures into the cryoelectron tomographic reconstruction of the intact human NPC and validating their placement with our nucleoporin interactome, we built a composite structure of the NPC symmetric core that contains ~320,000 residues and accounts for ~56 megadaltons of the NPC's structured mass. Our approach provides a paradigm for the structure determination of similarly complex macromolecular assemblies.


Architecture of the symmetric core of the nuclear pore.,Lin DH, Stuwe T, Schilbach S, Rundlet EJ, Perriches T, Mobbs G, Fan Y, Thierbach K, Huber FM, Collins LN, Davenport AM, Jeon YE, Hoelz A Science. 2016 Apr 15;352(6283):aaf1015. doi: 10.1126/science.aaf1015. Epub 2016, Apr 14. PMID:27081075<ref>PMID:27081075</ref>
==See Also==
 
*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5hb4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cbs 144 50]]
[[Category: Chaetomium thermophilum]]
[[Category: Hoelz, A]]
[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
[[Category: Lin, D H]]
[[Category: Large Structures]]
[[Category: Stuwe, T]]
[[Category: Hoelz A]]
[[Category: Nucleocytoplasmic transport]]
[[Category: Lin DH]]
[[Category: Protein transport]]
[[Category: Stuwe T]]
[[Category: Transport protein]]

Latest revision as of 15:31, 6 March 2024

Crystal structure of Chaetomium thermophilum Nup192Crystal structure of Chaetomium thermophilum Nup192

Structural highlights

5hb4 is a 1 chain structure with sequence from Chaetomium thermophilum and Chaetomium thermophilum var. thermophilum DSM 1495. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NU192_CHATD Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP192 is located to the NPC core at the nuclear membrane and is essential for de novo assembly of NPCs.[UniProtKB:P47054]

See Also

5hb4, resolution 3.20Å

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