4zsv: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4zsv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZSV FirstGlance]. <br>
<table><tr><td colspan='2'>[[4zsv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZSV FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zsv OCA], [https://pdbe.org/4zsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zsv RCSB], [https://www.ebi.ac.uk/pdbsum/4zsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zsv ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zsv OCA], [https://pdbe.org/4zsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zsv RCSB], [https://www.ebi.ac.uk/pdbsum/4zsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zsv ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/O67778_AQUAE O67778_AQUAE] [https://www.uniprot.org/uniprot/Q9I208_PSEAE Q9I208_PSEAE]  
[https://www.uniprot.org/uniprot/O67778_AQUAE O67778_AQUAE] [https://www.uniprot.org/uniprot/Q9I208_PSEAE Q9I208_PSEAE]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Connecting proteins together in prescribed geometric arrangements is an important element in new areas of biomolecular design. In this study, we characterize the degree of three-dimensional orientational control that can be achieved when two protein domains that have alpha-helical termini are joined using an alpha-helical linker. A fusion between naturally oligomeric protein domains was designed in this fashion with the intent of creating a self-assembling 12-subunit tetrahedral protein cage. While the designed fusion protein failed to assemble into a tetrahedral cage in high yield, a series of crystal structures showed that the two fused components were indeed bridged by an intact alpha helix, although the fusion protein was distorted from the intended ideal configuration by bending of the helix, ranging from 7 to 35 degrees . That range of deviation in orientation creates challenges for designing large, perfectly symmetric protein assemblies, although it should offer useful outcomes for other less geometrically demanding applications in synthetic biology.
On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.,Lai YT, Jiang L, Chen W, Yeates TO Protein Eng Des Sel. 2015 Aug 4. pii: gzv035. PMID:26243886<ref>PMID:26243886</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4zsv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 15:16, 6 March 2024

Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-1.0Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-1.0

Structural highlights

4zsv is a 2 chain structure with sequence from Aquifex aeolicus VF5 and Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 4.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O67778_AQUAE Q9I208_PSEAE

4zsv, resolution 4.20Å

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