4y9r: Difference between revisions

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 ==rat CYPOR mutant - G141del==
-<StructureSection load='4y9r' size='340' side='right' caption='[[4y9r]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='4y9r' size='340' side='right'caption='[[4y9r]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4y9r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y9R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4y9r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y9R FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4y9u|4y9u]], [[4yaf|4yaf]], [[4yal|4yal]], [[4yao|4yao]], [[4yau|4yau]], [[4yaw|4yaw]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH--hemoprotein_reductase NADPH--hemoprotein reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.4 1.6.2.4] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y9r OCA], [https://pdbe.org/4y9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y9r RCSB], [https://www.ebi.ac.uk/pdbsum/4y9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y9r ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y9r OCA], [http://pdbe.org/4y9r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y9r RCSB], [http://www.ebi.ac.uk/pdbsum/4y9r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y9r ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NCPR_RAT NCPR_RAT]] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
+[https://www.uniprot.org/uniprot/NCPR_RAT NCPR_RAT] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-NADPH-Cytochrome P450 oxidoreductase (CYPOR) transfers electrons from NADPH to cytochromes P450 via its FAD and FMN. To understand the biochemical and structural basis of electron transfer from FMN-hydroquinone to its partners, three deletion-mutants in a conserved loop near the FMN were characterized. Comparison of oxidized and reduced wild type and mutant structures reveals that the basis for the air-stability of the neutral-blue semiquinone is protonation of the flavin N5 and strong H-bond formation with the Gly-141 carbonyl. The Gly-143 protein had moderately decreased activity with cytochrome P450 and cytochrome c. It formed a flexible loop, which transiently interacts with the flavin N5, resulting in the generation of both an unstable neutral-blue semiquinone and hydroquinone. The Gly-141 and Gly-141/Glu-142Asn mutants were inactive with cytochrome P450, but fully active with reduced cytochrome c . In the Gly-141 mutants, the backbone amide of Glu/Asn 142 forms an H-bond to the N5 of the oxidized flavin, which leads to formation of an unstable red-anionic semiquinone with a more negative potential than the hydroquinone. The semiquinone of G141/E142N was slightly more stable than that of G141, consistent with its crystallographically demonstrated more rigid loop. Nonetheless, both Gly-141 red semiquinones were less stable than those of the corresponding loop in cytochrome P450 BM3 and the nNOS mutant (DeltaGly-810). Our results indicate that the catalytic activity of CYPOR is a function of the length, sequence, and flexibility of the 140s loop and illustrate the sophisticated variety of biochemical mechanisms employed in fine-tuning its redox properties and function.
-Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or Gly-143 Destabilize Its FMN Semiquinone.,Rwere F, Xia C, Im S, Haque MM, Stuehr DJ, Waskell L, Kim JP J Biol Chem. 2016 May 9. pii: jbc.M116.724625. PMID:27189945<ref>PMID:27189945</ref>
+==See Also==
+*[[NADPH-Cytochrome P450 Reductase|NADPH-Cytochrome P450 Reductase]]
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 4y9r" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: NADPH--hemoprotein reductase]]
+[[Category: Large Structures]]
-[[Category: Kim, J J.P]]
+[[Category: Rattus norvegicus]]
-[[Category: Xia, C]]
+[[Category: Kim JJP]]
-[[Category: Cytochrome p450 reductase]]
+[[Category: Xia C]]
-[[Category: Fmn binding]]
-[[Category: Oxidoreductase]]
-[[Category: Semiquinone]]