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==Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh1==
==Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh1==
<StructureSection load='4xzr' size='340' side='right' caption='[[4xzr]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
<StructureSection load='4xzr' size='340' side='right'caption='[[4xzr]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4xzr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XZR FirstGlance]. <br>
<table><tr><td colspan='2'>[[4xzr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XZR FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4pvz|4pvz]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xzr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xzr RCSB], [http://www.ebi.ac.uk/pdbsum/4xzr PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xzr OCA], [https://pdbe.org/4xzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xzr RCSB], [https://www.ebi.ac.uk/pdbsum/4xzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xzr ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SRC1_YEAST SRC1_YEAST]] Plays a role in sister chromatid separation.<ref>PMID:11754482</ref>  [[http://www.uniprot.org/uniprot/IMA1_YEAST IMA1_YEAST]] Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).<ref>PMID:7565597</ref> <ref>PMID:10913188</ref> <ref>PMID:21075847</ref> 
[https://www.uniprot.org/uniprot/SRC1_YEAST SRC1_YEAST] Plays a role in sister chromatid separation.<ref>PMID:11754482</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Targeting of ER-synthesized membrane proteins to the inner nuclear membrane (INM) has long been explained by the diffusion-retention model. However, several INM proteins contain non-classical nuclear localization signal (NLS) sequences, which, in a few instances, have been shown to promote importin alpha/beta- and Ran-dependent translocation to the INM. Here, using structural and biochemical methods, we show that yeast INM proteins Heh2 and Src1/Heh1 contain bipartite import sequences that associate intimately with the minor NLS-binding pocket of yeast importin alpha and unlike classical NLSs efficiently displace the IBB domain in the absence of importin beta. In vivo, the intimate interactions at the minor NLS-binding pocket make the h2NLS highly efficient at recruiting importin alpha at the ER and drive INM localization of endogenous Heh2. Thus, h1/h2NLSs delineate a novel class of super-potent, IBB-like membrane protein NLSs, distinct from classical NLSs found in soluble cargos and of general interest in biology.
 
Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2.,Lokareddy RK, Hapsari RA, van Rheenen M, Pumroy RA, Bhardwaj A, Steen A, Veenhoff LM, Cingolani G Structure. 2015 May 19. pii: S0969-2126(15)00175-6. doi:, 10.1016/j.str.2015.04.017. PMID:26051712<ref>PMID:26051712</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Importin 3D structures|Importin 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cingolani, G]]
[[Category: Large Structures]]
[[Category: Lokareddy, R K]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Karyopherin]]
[[Category: Cingolani G]]
[[Category: Membrane protein]]
[[Category: Lokareddy RK]]
[[Category: Nl]]
[[Category: Nuclear import]]

Latest revision as of 16:02, 1 March 2024

Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh1Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh1

Structural highlights

4xzr is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.25Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SRC1_YEAST Plays a role in sister chromatid separation.[1]

See Also

References

  1. ↑ Rodriguez-Navarro S, Igual JC, Perez-Ortin JE. SRC1: an intron-containing yeast gene involved in sister chromatid segregation. Yeast. 2002 Jan 15;19(1):43-54. PMID:11754482 doi:http://dx.doi.org/10.1002/yea.803

4xzr, resolution 2.25Å

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