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| ==Structure of Myc3 N-terminal JAZ-binding domain [44-238] in complex with Jas motif of JAZ9== | | ==Structure of Myc3 N-terminal JAZ-binding domain [44-238] in complex with Jas motif of JAZ9== |
| <StructureSection load='4rs9' size='340' side='right' caption='[[4rs9]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='4rs9' size='340' side='right'caption='[[4rs9]], [[Resolution|resolution]] 1.95Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4rs9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RS9 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4rs9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RS9 FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rqw|4rqw]], [[4rru|4rru]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rs9 OCA], [http://pdbe.org/4rs9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rs9 RCSB], [http://www.ebi.ac.uk/pdbsum/4rs9 PDBsum]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rs9 OCA], [https://pdbe.org/4rs9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rs9 RCSB], [https://www.ebi.ac.uk/pdbsum/4rs9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rs9 ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/MYC3_ARATH MYC3_ARATH]] Transcription factor involved in tryptophan, jasmonic acid (JA) and other stress-responsive gene regulation. With MYC2 and MYC4, controls additively subsets of JA-dependent responses. Can form complexes with all known glucosinolate-related MYBs to regulate glucosinolate biosynthesis. Binds to the G-box (5'-CACGTG-3') of promoters. Activates multiple TIFY/JAZ promoters.<ref>PMID:12136026</ref> <ref>PMID:21242320</ref> <ref>PMID:21321051</ref> <ref>PMID:21335373</ref> <ref>PMID:23943862</ref> [[http://www.uniprot.org/uniprot/TIF7_ARATH TIF7_ARATH]] Repressor of jasmonate responses negatively regulated by the proteasome in an SCF(COI1) E3 ubiquitin-protein ligase complex-dependent manner. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-TIFY7/JAZ9 interaction.<ref>PMID:19151223</ref> | | [https://www.uniprot.org/uniprot/TIF7_ARATH TIF7_ARATH] Repressor of jasmonate responses negatively regulated by the proteasome in an SCF(COI1) E3 ubiquitin-protein ligase complex-dependent manner. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-TIFY7/JAZ9 interaction.<ref>PMID:19151223</ref> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The plant hormone jasmonate plays crucial roles in regulating plant responses to herbivorous insects and microbial pathogens and is an important regulator of plant growth and development. Key mediators of jasmonate signalling include MYC transcription factors, which are repressed by jasmonate ZIM-domain (JAZ) transcriptional repressors in the resting state. In the presence of active jasmonate, JAZ proteins function as jasmonate co-receptors by forming a hormone-dependent complex with COI1, the F-box subunit of an SCF-type ubiquitin E3 ligase. The hormone-dependent formation of the COI1-JAZ co-receptor complex leads to ubiquitination and proteasome-dependent degradation of JAZ repressors and release of MYC proteins from transcriptional repression. The mechanism by which JAZ proteins repress MYC transcription factors and how JAZ proteins switch between the repressor function in the absence of hormone and the co-receptor function in the presence of hormone remain enigmatic. Here we show that Arabidopsis MYC3 undergoes pronounced conformational changes when bound to the conserved Jas motif of the JAZ9 repressor. The Jas motif, previously shown to bind to hormone as a partly unwound helix, forms a complete alpha-helix that displaces the amino (N)-terminal helix of MYC3 and becomes an integral part of the MYC N-terminal fold. In this position, the Jas helix competitively inhibits MYC3 interaction with the MED25 subunit of the transcriptional Mediator complex. Our structural and functional studies elucidate a dynamic molecular switch mechanism that governs the repression and activation of a major plant hormone pathway.
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| Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling.,Zhang F, Yao J, Ke J, Zhang L, Lam VQ, Xin XF, Zhou XE, Chen J, Brunzelle J, Griffin PR, Zhou M, Xu HE, Melcher K, He SY Nature. 2015 Aug 10. doi: 10.1038/nature14661. PMID:26258305<ref>PMID:26258305</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4rs9" style="background-color:#fffaf0;"></div>
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| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Brunzelle, J S]] | | [[Category: Arabidopsis thaliana]] |
| [[Category: He, S Y]] | | [[Category: Large Structures]] |
| [[Category: Ke, J]] | | [[Category: Brunzelle JS]] |
| [[Category: Melcher, K]] | | [[Category: He SY]] |
| [[Category: Xu, H E]] | | [[Category: Ke J]] |
| [[Category: Zhang, F]] | | [[Category: Melcher K]] |
| [[Category: Zhou, M]] | | [[Category: Xu HE]] |
| [[Category: Zhou, X E]] | | [[Category: Zhang F]] |
| [[Category: Alpha+beta structural fold]] | | [[Category: Zhou M]] |
| [[Category: Bind jaz corepressors or mediator subunit med25]] | | [[Category: Zhou XE]] |
| [[Category: Helix-sheet-helix]]
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| [[Category: Nuclear]]
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| [[Category: Transcription factor]]
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| [[Category: Transcription regulator]]
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