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==The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein==
==The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein==
<StructureSection load='4qb0' size='340' side='right' caption='[[4qb0]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
<StructureSection load='4qb0' size='340' side='right'caption='[[4qb0]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4qb0]] is a 1 chain structure. The October 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ebola Virus Proteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_10 10.2210/rcsb_pdb/mom_2014_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QB0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4qb0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976]. The October 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ebola Virus Proteins''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_10 10.2210/rcsb_pdb/mom_2014_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QB0 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qaz|4qaz]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qb0 RCSB], [http://www.ebi.ac.uk/pdbsum/4qb0 PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qb0 OCA], [https://pdbe.org/4qb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qb0 RCSB], [https://www.ebi.ac.uk/pdbsum/4qb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qb0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.  
[https://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ebolavirus (EBOV) causes severe hemorrhagic fever with a mortality rate of up to 90%. EBOV is a member of the order Mononegavirales and, like other viruses in this taxonomic group, contains a negative-sense single-stranded (ss) RNA. The EBOV ssRNA encodes seven distinct proteins. One of them, the nucleoprotein (NP), is the most abundant viral protein in the infected cell and within the viral nucleocapsid. Like other EBOV proteins, NP is multifunctional. It is tightly associated with the viral genome and is essential for viral transcription, RNA replication, genome packaging and nucleocapsid assembly prior to membrane encapsulation. NP is unusual among the Mononegavirales in that it contains two distinct regions, or putative domains, the C-terminal of which shows no homology to any known proteins and is purported to be a hub for protein-protein interactions within the nucleocapsid. The atomic structure of NP remains unknown. Here, the boundaries of the N- and C-terminal domains of NP from Zaire EBOV are defined, it is shown that they can be expressed as highly stable recombinant proteins in Escherichia coli, and the atomic structure of the C-terminal domain (residues 641-739) derived from analysis of two distinct crystal forms at 1.98 and 1.75 A resolution is described. The structure reveals a novel tertiary fold that is distantly reminiscent of the beta-grasp architecture.
 
The structure of the C-terminal domain of the Zaire ebolavirus nucleoprotein.,Dziubanska PJ, Derewenda U, Ellena JF, Engel DA, Derewenda ZS Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2420-9. doi:, 10.1107/S1399004714014710. Epub 2014 Aug 29. PMID:25195755<ref>PMID:25195755</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ebola Virus Proteins]]
[[Category: Ebola Virus Proteins]]
[[Category: Ebola virus - Mayinga, Zaire, 1976]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Derewenda, U]]
[[Category: Derewenda U]]
[[Category: Derewenda, Z S]]
[[Category: Derewenda ZS]]
[[Category: Dziubanska, P J]]
[[Category: Dziubanska PJ]]
[[Category: Ebola virus vp40]]
[[Category: New family]]
[[Category: Viral protein]]

Latest revision as of 15:45, 1 March 2024

The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoproteinThe crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein

Structural highlights

4qb0 is a 1 chain structure with sequence from Ebola virus - Mayinga, Zaire, 1976. The October 2014 RCSB PDB Molecule of the Month feature on Ebola Virus Proteins by David Goodsell is 10.2210/rcsb_pdb/mom_2014_10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCAP_EBOZM Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.

See Also

4qb0, resolution 1.75Å

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