4ojt: Difference between revisions

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New page: '''Unreleased structure''' The entry 4ojt is ON HOLD Authors: Mishra, V., Ronning, D.R. Description: Helicobacter pylori MTAN complexed with S-ribosylhomocysteine and adenine
 
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'''Unreleased structure'''


The entry 4ojt is ON HOLD
==Helicobacter pylori MTAN complexed with S-ribosylhomocysteine and adenine==
 
<StructureSection load='4ojt' size='340' side='right'caption='[[4ojt]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
Authors: Mishra, V., Ronning, D.R.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[4ojt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OJT FirstGlance]. <br>
Description: Helicobacter pylori MTAN complexed with S-ribosylhomocysteine and adenine
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2WP:(2S)-2-AMINO-4-({[(2S,3S,4R,5S)-3,4,5-TRIHYDROXYTETRAHYDROFURAN-2-YL]METHYL}SULFANYL)BUTANOIC+ACID'>2WP</scene>, <scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ojt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ojt OCA], [https://pdbe.org/4ojt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ojt RCSB], [https://www.ebi.ac.uk/pdbsum/4ojt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ojt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MQMTN_HELPJ MQMTN_HELPJ] Catalyzes the direct conversion of aminodeoxyfutalosine (AFL) into dehypoxanthine futalosine (DHFL) and adenine via the hydrolysis of the N-glycosidic bond; this reaction seems to represent an essential step in the menaquinone biosynthesis pathway in Helicobacter species. Also catalyzes the hydrolysis of 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose. Can also probably use S-adenosylhomocysteine (SAH) as substrate, leading to adenine and S-ribosylhomocysteine. These other activities highlight the tremendous versatility of the enzyme, which also plays key roles in S-adenosylmethionine recycling and in the biosynthesis of the quorum-sensing molecule autoinducer-2.<ref>PMID:20954236</ref> <ref>PMID:22891633</ref>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Helicobacter pylori J99]]
[[Category: Large Structures]]
[[Category: Mishra V]]
[[Category: Ronning DR]]

Latest revision as of 15:40, 1 March 2024

Helicobacter pylori MTAN complexed with S-ribosylhomocysteine and adenineHelicobacter pylori MTAN complexed with S-ribosylhomocysteine and adenine

Structural highlights

4ojt is a 1 chain structure with sequence from Helicobacter pylori J99. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MQMTN_HELPJ Catalyzes the direct conversion of aminodeoxyfutalosine (AFL) into dehypoxanthine futalosine (DHFL) and adenine via the hydrolysis of the N-glycosidic bond; this reaction seems to represent an essential step in the menaquinone biosynthesis pathway in Helicobacter species. Also catalyzes the hydrolysis of 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose. Can also probably use S-adenosylhomocysteine (SAH) as substrate, leading to adenine and S-ribosylhomocysteine. These other activities highlight the tremendous versatility of the enzyme, which also plays key roles in S-adenosylmethionine recycling and in the biosynthesis of the quorum-sensing molecule autoinducer-2.[1] [2]

References

  1. ↑ Ronning DR, Iacopelli NM, Mishra V. Enzyme-ligand interactions that drive active site rearrangements in the Helicobacter pylori 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase. Protein Sci. 2010 Oct 15. PMID:20954236 doi:10.1002/pro.524
  2. ↑ Wang S, Haapalainen AM, Yan F, Du Q, Tyler PC, Evans GB, Rinaldo-Matthis A, Brown RL, Norris GE, Almo SC, Schramm VL. A Picomolar Transition State Analogue Inhibitor of MTAN as a Specific Antibiotic for Helicobacter pylori. Biochemistry. 2012 Sep 4;51(35):6892-4. Epub 2012 Aug 22. PMID:22891633 doi:http://dx.doi.org/10.1021/bi3009664

4ojt, resolution 1.50Å

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