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== | ==Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii== | ||
[[4oge]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGE OCA]. | <StructureSection load='4oge' size='340' side='right'caption='[[4oge]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4oge]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomyces_naeslundii_str._Howell_279 Actinomyces naeslundii str. Howell 279]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OGE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.201Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oge OCA], [https://pdbe.org/4oge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oge RCSB], [https://www.ebi.ac.uk/pdbsum/4oge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oge ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CAS9_ACTNH CAS9_ACTNH] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity).<ref>PMID:24505130</ref> | |||
== | ==See Also== | ||
*[[CRISPR-Cas9 Part II|CRISPR-Cas9 Part II]] | |||
[[ | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | ||
[[ | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: | [[Category: Actinomyces naeslundii str. Howell 279]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Doudna JA]] | ||
[[Category: | [[Category: Jiang F]] | ||
[[Category: | [[Category: Lin S]] | ||
[[Category: | [[Category: Ma E]] | ||
Latest revision as of 15:39, 1 March 2024
Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundiiCrystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii
Structural highlights
FunctionCAS9_ACTNH CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity).[1] See AlsoReferences
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