4mih: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
{{Large structure}}
 
==Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant==
==Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant==
<StructureSection load='4mih' size='340' side='right' caption='[[4mih]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='4mih' size='340' side='right'caption='[[4mih]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4mih]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysosporium_pruinosum_(gilman_&_abbott)_carmich. Chrysosporium pruinosum (gilman & abbott) carmich.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MIH FirstGlance]. <br>
<table><tr><td colspan='2'>[[4mih]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MIH FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=G3F:3-DEOXY-3-FLUORO-BETA-D-GLUCOPYRANOSE'>G3F</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mif|4mif]], [[4mig|4mig]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=G3F:3-DEOXY-3-FLUORO-BETA-D-GLUCOPYRANOSE'>G3F</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">p2o, p2ox, pox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5306 Chrysosporium pruinosum (Gilman & Abbott) Carmich.])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mih OCA], [https://pdbe.org/4mih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mih RCSB], [https://www.ebi.ac.uk/pdbsum/4mih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mih ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyranose_oxidase Pyranose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.10 1.1.3.10] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mih OCA], [http://pdbe.org/4mih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mih RCSB], [http://www.ebi.ac.uk/pdbsum/4mih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mih ProSAT]</span></td></tr>
</table>
</table>
{{Large structure}}
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/P2OX_PHACH P2OX_PHACH]] Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.<ref>PMID:9210340</ref> <ref>PMID:8661938</ref> 
[https://www.uniprot.org/uniprot/P2OX_PHACH P2OX_PHACH] Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.<ref>PMID:9210340</ref> <ref>PMID:8661938</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The flavin-dependent homotetrameric enzyme pyranose 2-oxidase (P2O) is found mostly, but not exclusively, in lignocellulose-degrading fungi where it catalyzes the oxidation of beta-d-glucose to the corresponding 2-keto sugar concomitantly with hydrogen peroxide formation during lignin solubilization. Here, we present crystal structures of P2O from the efficient lignocellulolytic basidiomycete Phanerochaete chrysosporium. Structures were determined of wild-type PcP2O from the natural fungal source, and two variants of recombinant full-length PcP2O, both in complex with the slow substrate 3-deoxy-3-fluoro-beta-d-glucose. The active sites in PcP2O and P2O from Trametes multicolor (TmP2O) are highly conserved with identical substrate binding. Our structural analysis suggests that the 17 degrees C higher melting temperature of PcP2O compared to TmP2O is due to an increased number of intersubunit salt bridges. The structure of recombinant PcP2O expressed with its natural N-terminal sequence, including a proposed propeptide segment, reveals that the first five residues of the propeptide intercalate at the interface between A and B subunits to form stabilizing, mainly hydrophobic, interactions. In the structure of mature PcP2O purified from the natural source, the propeptide segment in subunit A has been replaced by a nearby loop in the B subunit. We propose that the propeptide in subunit A stabilizes the A/B interface of essential dimers in the homotetramer and that, upon maturation, it is replaced by the loop in the B subunit to form the mature subunit interface. This would imply that the propeptide segment of PcP2O acts as an intramolecular chaperone for oligomerization at the A/B interface of the essential dimer.
 
Crystal structures of pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly.,Hassan N, Tan TC, Spadiut O, Pisanelli I, Fusco L, Haltrich D, Peterbauer CK, Divne C FEBS Open Bio. 2013 Nov 5;3:496-504. PMID:24282677<ref>PMID:24282677</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Pyranose oxidase|Pyranose oxidase]]
<div class="pdbe-citations 4mih" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pyranose oxidase]]
[[Category: Large Structures]]
[[Category: Divne, C]]
[[Category: Phanerodontia chrysosporium]]
[[Category: Fusco, L]]
[[Category: Divne C]]
[[Category: Haltrich, D]]
[[Category: Fusco L]]
[[Category: Hassan, N]]
[[Category: Haltrich D]]
[[Category: Peterbauer, C]]
[[Category: Hassan N]]
[[Category: Pisanelli, I]]
[[Category: Peterbauer C]]
[[Category: Spadiut, O]]
[[Category: Pisanelli I]]
[[Category: Tan, T C]]
[[Category: Spadiut O]]
[[Category: Flavinylation]]
[[Category: Tan TC]]
[[Category: Gmc oxidoreductase]]
[[Category: Homotetramer]]
[[Category: Hyphae]]
[[Category: Oxidoreductase]]
[[Category: Phbh fold]]
[[Category: Pyranose 2-oxidaser oxidoreductase]]
[[Category: Rossmann fold]]

Latest revision as of 15:26, 1 March 2024

Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutantPyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant

Structural highlights

4mih is a 8 chain structure with sequence from Phanerodontia chrysosporium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P2OX_PHACH Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.[1] [2]

See Also

References

  1. ↑ Artolozaga MJ, Kubatova E, Volc J, Kalisz HM. Pyranose 2-oxidase from Phanerochaete chrysosporium--further biochemical characterisation. Appl Microbiol Biotechnol. 1997 May;47(5):508-14. PMID:9210340
  2. ↑ Volc J, Kubatova E, Daniel G, Prikrylova V. Only C-2 specific glucose oxidase activity is expressed in ligninolytic cultures of the white rot fungus Phanerochaete chrysosporium. Arch Microbiol. 1996 Jun;165(6):421-4. PMID:8661938

4mih, resolution 2.40Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=4mih&oldid=4075324"