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{{Large structure}}
{{STRUCTURE_4mih|  PDB=4mih  |  SCENE=  }}
===Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant===
{{ABSTRACT_PUBMED_24282677}}


==Function==
==Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant==
[[http://www.uniprot.org/uniprot/P2OX_PHACH P2OX_PHACH]] Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.<ref>PMID:9210340</ref> <ref>PMID:8661938</ref>
<StructureSection load='4mih' size='340' side='right'caption='[[4mih]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4mih]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MIH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=G3F:3-DEOXY-3-FLUORO-BETA-D-GLUCOPYRANOSE'>G3F</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mih OCA], [https://pdbe.org/4mih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mih RCSB], [https://www.ebi.ac.uk/pdbsum/4mih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mih ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/P2OX_PHACH P2OX_PHACH] Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.<ref>PMID:9210340</ref> <ref>PMID:8661938</ref>  


==About this Structure==
==See Also==
[[4mih]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIH OCA].
*[[Pyranose oxidase|Pyranose oxidase]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024282677</ref><references group="xtra"/><references/>
__TOC__
[[Category: Pyranose oxidase]]
</StructureSection>
[[Category: Divne, C.]]
[[Category: Large Structures]]
[[Category: Fusco, L.]]
[[Category: Phanerodontia chrysosporium]]
[[Category: Haltrich, D.]]
[[Category: Divne C]]
[[Category: Hassan, N.]]
[[Category: Fusco L]]
[[Category: Peterbauer, C.]]
[[Category: Haltrich D]]
[[Category: Pisanelli, I.]]
[[Category: Hassan N]]
[[Category: Spadiut, O.]]
[[Category: Peterbauer C]]
[[Category: Tan, T C.]]
[[Category: Pisanelli I]]
[[Category: Flavinylation]]
[[Category: Spadiut O]]
[[Category: Gmc oxidoreductase]]
[[Category: Tan TC]]
[[Category: Homotetramer]]
[[Category: Hyphae]]
[[Category: Oxidoreductase]]
[[Category: Phbh fold]]
[[Category: Pyranose 2-oxidaser oxidoreductase]]
[[Category: Rossmann fold]]

Latest revision as of 15:26, 1 March 2024

Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutantPyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant

Structural highlights

4mih is a 8 chain structure with sequence from Phanerodontia chrysosporium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P2OX_PHACH Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.[1] [2]

See Also

References

  1. ↑ Artolozaga MJ, Kubatova E, Volc J, Kalisz HM. Pyranose 2-oxidase from Phanerochaete chrysosporium--further biochemical characterisation. Appl Microbiol Biotechnol. 1997 May;47(5):508-14. PMID:9210340
  2. ↑ Volc J, Kubatova E, Daniel G, Prikrylova V. Only C-2 specific glucose oxidase activity is expressed in ligninolytic cultures of the white rot fungus Phanerochaete chrysosporium. Arch Microbiol. 1996 Jun;165(6):421-4. PMID:8661938

4mih, resolution 2.40Å

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