4mi7: Difference between revisions

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 ==Crystal Structure of Salmonella Effector Protein GtgE==
-<StructureSection load='4mi7' size='340' side='right' caption='[[4mi7]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='4mi7' size='340' side='right'caption='[[4mi7]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salts Salts]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MI7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._SL1344 Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MI7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gtgE, SL1344_0995 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216597 SALTS])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mi7 OCA], [http://pdbe.org/4mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/4mi7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mi7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mi7 OCA], [https://pdbe.org/4mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/4mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mi7 ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/H9L447_SALTY H9L447_SALTY]
-GtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 A resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease inhibitors were examined and it was determined that N-ethylmaleimide, antipain and chymostatin inhibit GtgE activity in vitro. These findings provide the basis for the development of novel therapeutic strategies to combat Salmonella infections.
-Structural and enzymatic characterization of a host-specificity determinant from Salmonella.,Kohler AC, Spano S, Galan JE, Stebbins CE Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):384-91. doi:, 10.1107/S1399004713028393. Epub 2014 Jan 29. PMID:24531472<ref>PMID:24531472</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 4mi7" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Salts]]
+[[Category: Large Structures]]
-[[Category: Kohler, A C]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]]
-[[Category: Stebbins, C E]]
+[[Category: Kohler AC]]
-[[Category: Bacterial protein]]
+[[Category: Stebbins CE]]
-[[Category: Hydrolase]]
-[[Category: Toxin]]