|
|
| (2 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
|
| |
|
| ==Structure of the THF riboswitch bound to 2,6-diaminopurine== | | ==Structure of the THF riboswitch bound to 2,6-diaminopurine== |
| <StructureSection load='4lvz' size='340' side='right' caption='[[4lvz]], [[Resolution|resolution]] 1.77Å' scene=''> | | <StructureSection load='4lvz' size='340' side='right'caption='[[4lvz]], [[Resolution|resolution]] 1.77Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4lvz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LVZ FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4lvz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LVZ FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6AP:9H-PURINE-2,6-DIAMINE'>6AP</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd3|3sd3]], [[4lvv|4lvv]], [[4lvw|4lvw]], [[4lvy|4lvy]], [[4lvx|4lvx]], [[4lw0|4lw0]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6AP:9H-PURINE-2,6-DIAMINE'>6AP</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvz OCA], [http://pdbe.org/4lvz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lvz RCSB], [http://www.ebi.ac.uk/pdbsum/4lvz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvz OCA], [https://pdbe.org/4lvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lvz RCSB], [https://www.ebi.ac.uk/pdbsum/4lvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvz ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| The tetrahydrofolate (THF) riboswitch regulates folate transport and metabolism in a number of Firmicutes by cooperatively binding two molecules of THF. To further understand this riboswitch's specificity for THF, binding and regulatory activity of a series of THF analogs and antifolates were examined. Our data reveal that although binding is dominated by the RNA's interactions with the pterin moiety, the para-aminobenzoic acid (pABA) moiety plays a significant role in transcriptional regulation. Further, we find that adenine and several other analogs bind with high affinity by an alternative binding mechanism. Despite a similar affinity to THF, adenine is a poor regulator of transcriptional attenuation. These results demonstrate that binding alone does not determine a compound's effectiveness in regulating the activity of the riboswitch-a complication in current efforts to develop antimicrobials that target these RNAs.
| |
|
| |
| A Disconnect between High-Affinity Binding and Efficient Regulation by Antifolates and Purines in the Tetrahydrofolate Riboswitch.,Trausch JJ, Batey RT Chem Biol. 2014 Feb 20;21(2):205-16. doi: 10.1016/j.chembiol.2013.11.012. Epub, 2014 Jan 2. PMID:24388757<ref>PMID:24388757</ref>
| |
|
| |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 4lvz" style="background-color:#fffaf0;"></div>
| |
|
| |
|
| ==See Also== | | ==See Also== |
| *[[Riboswitch|Riboswitch]] | | *[[Riboswitch 3D structures|Riboswitch 3D structures]] |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Batey, R T]] | | [[Category: Large Structures]] |
| [[Category: Trausch, J J]]
| | [[Category: Streptococcus mutans]] |
| [[Category: 6-diaminopurine]]
| | [[Category: Batey RT]] |
| [[Category: Aptamer]]
| | [[Category: Trausch JJ]] |
| [[Category: Bacillus subtili]]
| |
| [[Category: Bacterial]]
| |
| [[Category: Bacterial protein]]
| |
| [[Category: Base sequence]]
| |
| [[Category: Binding site]]
| |
| [[Category: Calorimetry]]
| |
| [[Category: Folic acid]]
| |
| [[Category: Gene expression regulation]]
| |
| [[Category: Genetic]]
| |
| [[Category: Guanine]]
| |
| [[Category: Leucovorin]]
| |
| [[Category: Ligand]]
| |
| [[Category: Magnesium]]
| |
| [[Category: Molecular sequence data]]
| |
| [[Category: Mrna]]
| |
| [[Category: Ncrna]]
| |
| [[Category: Nucleic acid conformation]]
| |
| [[Category: Nucleotide]]
| |
| [[Category: Point mutation]]
| |
| [[Category: Protein binding]]
| |
| [[Category: Protein structure]]
| |
| [[Category: Pseudoknot]]
| |
| [[Category: Regulation]]
| |
| [[Category: Riboswitch]]
| |
| [[Category: Rna]]
| |
| [[Category: S-adenosylmethionine]]
| |
| [[Category: Secondary]]
| |
| [[Category: Streptococcus mutan]] | |
| [[Category: Terminator region]] | |
| [[Category: Tetrahydrofolate]] | |
| [[Category: Thermodynamic]]
| |
| [[Category: Three-way junction]]
| |
| [[Category: Transcription]]
| |