4lni: Difference between revisions

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-{{Large structure}}
-{{STRUCTURE_4lni|  PDB=4lni  |  SCENE=  }}
-===B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex===
-{{ABSTRACT_PUBMED_24158439}}
-==About this Structure==
+==B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex==
-[[4lni]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LNI OCA].
+<StructureSection load='4lni' size='340' side='right'caption='[[4lni]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4lni]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LNI FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5793&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lni OCA], [https://pdbe.org/4lni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lni RCSB], [https://www.ebi.ac.uk/pdbsum/4lni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lni ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GLN1A_BACSU GLN1A_BACSU] Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism (PubMed:25691471). It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia (PubMed:24158439). Feedback-inhibited GlnA interacts with and regulates the activity of the transcriptional regulator TnrA (PubMed:11719184, PubMed:12139611). During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation (PubMed:11719184, PubMed:12139611, PubMed:25691471). Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity (PubMed:11719184, PubMed:12139611, PubMed:25691471). In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes (PubMed:25691471).<ref>PMID:11719184</ref> <ref>PMID:12139611</ref> <ref>PMID:24158439</ref> <ref>PMID:25691471</ref>
-==Reference==
+==See Also==
-<ref group="xtra">PMID:024158439</ref><references group="xtra"/><references/>
+*[[Glutamine synthetase 3D structures|Glutamine synthetase 3D structures]]
-[[Category: Bacillus globigii migula 1900]]
+== References ==
-[[Category: Glutamate--ammonia ligase]]
+<references/>
-[[Category: Chinnam, N.]]
+__TOC__
-[[Category: Fisher, S.]]
+</StructureSection>
-[[Category: Schumacher, M A.]]
+[[Category: Bacillus subtilis]]
-[[Category: Tonthat, N.]]
+[[Category: Large Structures]]
-[[Category: Wray, L.]]
+[[Category: Chinnam N]]
-[[Category: Alpha-beta]]
+[[Category: Fisher S]]
-[[Category: Glnra]]
+[[Category: Schumacher MA]]
-[[Category: Ligase]]
+[[Category: Tonthat N]]
-[[Category: Tnra]]
+[[Category: Wray L]]