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<StructureSection load='4jgi' size='340' side='right'caption='[[4jgi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='4jgi' size='340' side='right'caption='[[4jgi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4jgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desha Desha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JGI FirstGlance]. <br>
<table><tr><td colspan='2'>[[4jgi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfitobacterium_hafniense Desulfitobacterium hafniense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JGI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COB:CO-METHYLCOBALAMIN'>COB</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jgi OCA], [http://pdbe.org/4jgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jgi RCSB], [http://www.ebi.ac.uk/pdbsum/4jgi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jgi ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COB:CO-METHYLCOBALAMIN'>COB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jgi OCA], [https://pdbe.org/4jgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jgi RCSB], [https://www.ebi.ac.uk/pdbsum/4jgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jgi ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q8RPG0_DESHA Q8RPG0_DESHA]
This study describes the identification and the structural and spectroscopic analysis of a cobalamin-binding protein (termed CobDH) implicated in O-demethylation by the organohalide-respiring bacterium Desulfitobacterium hafniense DCB-2. The 1.5 A resolution crystal structure of CobDH is presented in the cobalamin-bound state and reveals that the protein is composed of an N-terminal helix-bundle domain and a C-terminal Rossmann-fold domain, with the cobalamin coordinated in the base-off/His-on conformation similar to other cobalamin-binding domains that catalyse methyl-transfer reactions. EPR spectroscopy of CobDH confirms cobalamin binding and reveals the presence of a cob(III)alamin superoxide, indicating binding of oxygen to the fully oxidized cofactor. These data provide the first structural insights into the methyltransferase reactions that occur during O-demethylation by D. hafniense.
 
Structure of the cobalamin-binding protein of a putative O-demethylase from Desulfitobacterium hafniense DCB-2.,Sjuts H, Dunstan MS, Fisher K, Leys D Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1609-16. doi:, 10.1107/S0907444913011323. Epub 2013 Jul 20. PMID:23897483<ref>PMID:23897483</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4jgi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Desha]]
[[Category: Desulfitobacterium hafniense]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Dunstan, M S]]
[[Category: Dunstan MS]]
[[Category: Fisher, K]]
[[Category: Fisher K]]
[[Category: Leys, D]]
[[Category: Leys D]]
[[Category: Sjuts, H]]
[[Category: Sjuts H]]
[[Category: Cobalamin]]
[[Category: Cobalamin binding]]
[[Category: Protein binding]]
[[Category: Rossmann fold]]

Latest revision as of 15:03, 1 March 2024

1.5 Angstrom crystal structure of a novel cobalamin-binding protein from Desulfitobacterium hafniense DCB-21.5 Angstrom crystal structure of a novel cobalamin-binding protein from Desulfitobacterium hafniense DCB-2

Structural highlights

4jgi is a 2 chain structure with sequence from Desulfitobacterium hafniense. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8RPG0_DESHA

4jgi, resolution 1.50Å

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