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| ==Crystal structure of the T1L reovirus attachment protein sigma1 in complex with alpha-2,3-sialyllactose== | | ==Crystal structure of the T1L reovirus attachment protein sigma1 in complex with alpha-2,3-sialyllactose== |
| <StructureSection load='4gu4' size='340' side='right' caption='[[4gu4]], [[Resolution|resolution]] 3.50Å' scene=''> | | <StructureSection load='4gu4' size='340' side='right'caption='[[4gu4]], [[Resolution|resolution]] 3.50Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4gu4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_1_lang Mammalian orthoreovirus 1 lang]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GU4 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4gu4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_1_Lang Mammalian orthoreovirus 1 Lang]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GU4 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3s6x|3s6x]], [[1kke|1kke]], [[4gu3|4gu3]]</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gu4 OCA], [https://pdbe.org/4gu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gu4 RCSB], [https://www.ebi.ac.uk/pdbsum/4gu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gu4 ProSAT]</span></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10884 Mammalian orthoreovirus 1 Lang])</td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gu4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gu4 RCSB], [http://www.ebi.ac.uk/pdbsum/4gu4 PDBsum]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/SIGM1_REOVL SIGM1_REOVL]] Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis (By similarity). | | [https://www.uniprot.org/uniprot/SIGM1_REOVL SIGM1_REOVL] Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis (By similarity). |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Viral attachment to target cells is the first step in infection and also serves as a determinant of tropism. Like many viruses, mammalian reoviruses bind with low affinity to cell-surface carbohydrate receptors to initiate the infectious process. Reoviruses disseminate with serotype-specific tropism in the host, which may be explained by differential glycan utilization. Although alpha2,3-linked sialylated oligosaccharides serve as carbohydrate receptors for type 3 reoviruses, neither a specific glycan bound by any reovirus serotype nor the function of glycan binding in type 1 reovirus infection was known. We have identified the oligosaccharide portion of ganglioside GM2 (the GM2 glycan) as a receptor for the attachment protein sigma1 of reovirus strain type 1 Lang (T1L) using glycan array screening. The interaction of T1L sigma1 with GM2 in solution was confirmed using NMR spectroscopy. We established that GM2 glycan engagement is required for optimal infection of mouse embryonic fibroblasts (MEFs) by T1L. Preincubation with GM2 specifically inhibited type 1 but not type 3 reovirus infection of MEFs. To provide a structural basis for these observations, we defined the mode of receptor recognition by determining the crystal structure of T1L sigma1 in complex with the GM2 glycan. GM2 binds in a shallow groove in the globular head domain of T1L sigma1. Both terminal sugar moieties of the GM2 glycan, N-acetylneuraminic acid and N-acetylgalactosamine, form contacts with the protein, providing an explanation for the observed specificity for GM2. Viruses with mutations in the glycan-binding domain display diminished hemagglutination capacity, a property dependent on glycan binding, and reduced capacity to infect MEFs. Our results define a novel mode of virus-glycan engagement and provide a mechanistic explanation for the serotype-dependent differences in glycan utilization by reovirus.
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| The GM2 Glycan Serves as a Functional Coreceptor for Serotype 1 Reovirus.,Reiss K, Stencel JE, Liu Y, Blaum BS, Reiter DM, Feizi T, Dermody TS, Stehle T PLoS Pathog. 2012 Dec;8(12):e1003078. doi: 10.1371/journal.ppat.1003078. Epub, 2012 Dec 6. PMID:23236285<ref>PMID:23236285</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| | ==See Also== |
| </div>
| | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Mammalian orthoreovirus 1 lang]] | | [[Category: Large Structures]] |
| [[Category: Reiss, K]] | | [[Category: Mammalian orthoreovirus 1 Lang]] |
| [[Category: Stehle, T]] | | [[Category: Reiss K]] |
| [[Category: 3-sialyllactose junctional adhesion molecule some]]
| | [[Category: Stehle T]] |
| [[Category: Beta-barrel]]
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| [[Category: Beta-spiral repeat]]
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| [[Category: Gm2 glycan alpha-2]]
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| [[Category: Greek key motif]]
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| [[Category: Triple beta-spiral]]
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| [[Category: Viral attachment protein]]
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| [[Category: Viral capsid]]
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| [[Category: Viral protein]]
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