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==Apo Structure of a Thiazolinyl Imine Reductase from Yersinia enterocolitica (Irp3)==
==Apo Structure of a Thiazolinyl Imine Reductase from Yersinia enterocolitica (Irp3)==
<StructureSection load='4gmf' size='340' side='right' caption='[[4gmf]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
<StructureSection load='4gmf' size='340' side='right'caption='[[4gmf]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4gmf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica_subsp._enterocolitica_8081 Yersinia enterocolitica subsp. enterocolitica 8081]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GMF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GMF FirstGlance]. <br>
<table><tr><td colspan='2'>[[4gmf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica_subsp._enterocolitica_8081 Yersinia enterocolitica subsp. enterocolitica 8081]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GMF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gmg|4gmg]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Irp3, ybtU, YE2619 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=393305 Yersinia enterocolitica subsp. enterocolitica 8081])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gmf OCA], [https://pdbe.org/4gmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gmf RCSB], [https://www.ebi.ac.uk/pdbsum/4gmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gmf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gmf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gmf RCSB], [http://www.ebi.ac.uk/pdbsum/4gmf PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A1JTG0_YERE8 A1JTG0_YERE8]
The thiazolinyl imine reductase from Yersinia enterocolitica (Irp3) catalyzes the NADPH-dependent reduction of a thiazoline ring in an intermediate for the formation of the siderophore yersiniabactin. Two structures of Irp3 were determined in the apo (1.85 A) and NADP(+)-bound (2.31 A) forms. Irp3 is structurally homologous to sugar oxidoreductases such as glucose-fructose oxidoreductase and 1,5-anhydro-d-fructose reductase, as well as to biliverdin reductase. A homology model of the thiazolinyl imine reductase from Pseudomonas aeruginosa (PchG) was generated. Extensive loop insertions are observed in the C-terminal domain that are unique to Irp3 and PchG and not found in the structural homologues that recognize small molecular substrates. These loops are hypothesized to be important for binding of the nonribosomal peptide synthetase modules (found in HMWP1 and PchF, respectively) to which the substrate of the reductase is covalently attached. A catalytic mechanism for the donation of a proton from a general acid (either histidine 101 or tyrosine 128) and the donation of a hydride from C4 of nicotinamide of the NADPH cofactor is proposed for reduction of the carbon-nitrogen double bond of the thiazoline.
 
Two Structures of a Thiazolinyl Imine Reductase from Yersinia enterocolitica Provide Insight into Catalysis and Binding to the Nonribosomal Peptide Synthetase Module of HMWP1.,Meneely KM, Lamb AL Biochemistry. 2012 Nov 6;51(44):9002-13. doi: 10.1021/bi3011016. Epub 2012 Oct, 23. PMID:23066849<ref>PMID:23066849</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Yersinia enterocolitica subsp. enterocolitica 8081]]
[[Category: Yersinia enterocolitica subsp. enterocolitica 8081]]
[[Category: Lamb, A L]]
[[Category: Lamb AL]]
[[Category: Meneely, K M]]
[[Category: Meneely KM]]
[[Category: Nadph dependent thiazoline reductase]]
[[Category: Oxidoreductase]]
[[Category: Rossmann fold]]

Latest revision as of 14:32, 1 March 2024

Apo Structure of a Thiazolinyl Imine Reductase from Yersinia enterocolitica (Irp3)Apo Structure of a Thiazolinyl Imine Reductase from Yersinia enterocolitica (Irp3)

Structural highlights

4gmf is a 4 chain structure with sequence from Yersinia enterocolitica subsp. enterocolitica 8081. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.85Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A1JTG0_YERE8

4gmf, resolution 1.85Å

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OCA
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