4ff4: Difference between revisions

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'''Unreleased structure'''


The entry 4ff4 is ON HOLD  until Paper Publication
==N4 mini-vRNAP transcription initiation complex, 4 min after soaking GTP, ATP and Mn==
<StructureSection load='4ff4' size='340' side='right'caption='[[4ff4]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ff4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N4 Escherichia virus N4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FF4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.026&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ff4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ff4 OCA], [https://pdbe.org/4ff4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ff4 RCSB], [https://www.ebi.ac.uk/pdbsum/4ff4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ff4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOLV_BPN4 RPOLV_BPN4] DNA-dependent RNA polymerase, which is injected into the host upon infection and transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure.<ref>PMID:18362338</ref> <ref>PMID:19061645</ref>


Authors: Murakami, K.S.
==See Also==
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
Description: N4 mini-vRNAP transcription initiation complex, 4 min after soaking GTP, ATP and Mn
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia virus N4]]
[[Category: Large Structures]]
[[Category: Murakami KS]]

Latest revision as of 14:15, 1 March 2024

N4 mini-vRNAP transcription initiation complex, 4 min after soaking GTP, ATP and MnN4 mini-vRNAP transcription initiation complex, 4 min after soaking GTP, ATP and Mn

Structural highlights

4ff4 is a 4 chain structure with sequence from Escherichia virus N4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.026Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPOLV_BPN4 DNA-dependent RNA polymerase, which is injected into the host upon infection and transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure.[1] [2]

See Also

References

  1. ↑ Murakami KS, Davydova EK, Rothman-Denes LB. X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase. Proc Natl Acad Sci U S A. 2008 Mar 24;. PMID:18362338 doi:0712325105
  2. ↑ Gleghorn ML, Davydova EK, Rothman-Denes LB, Murakami KS. Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase. Mol Cell. 2008 Dec 5;32(5):707-17. PMID:19061645 doi:S1097-2765(08)00777-6

4ff4, resolution 2.03Å

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