4f9b: Difference between revisions

Latest revision as of 14:12, 1 March 2024

Human CDC7 kinase in complex with DBF4 and PHA767491Human CDC7 kinase in complex with DBF4 and PHA767491

Structural highlights

4f9b is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DBF4A_HUMAN Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.^[1] ^[2] ^[3]

References

↑ Kumagai H, Sato N, Yamada M, Mahony D, Seghezzi W, Lees E, Arai K, Masai H. A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells. Mol Cell Biol. 1999 Jul;19(7):5083-95. PMID:10373557
↑ Jiang W, McDonald D, Hope TJ, Hunter T. Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication. EMBO J. 1999 Oct 15;18(20):5703-13. PMID:10523313 doi:http://dx.doi.org/10.1093/emboj/18.20.5703
↑ Tenca P, Brotherton D, Montagnoli A, Rainoldi S, Albanese C, Santocanale C. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem. 2007 Jan 5;282(1):208-15. Epub 2006 Oct 24. PMID:17062569 doi:http://dx.doi.org/10.1074/jbc.M604457200

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OCA

[1] Kumagai H, Sato N, Yamada M, Mahony D, Seghezzi W, Lees E, Arai K, Masai H. A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells. Mol Cell Biol. 1999 Jul;19(7):5083-95. PMID:10373557

[2] Jiang W, McDonald D, Hope TJ, Hunter T. Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication. EMBO J. 1999 Oct 15;18(20):5703-13. PMID:10523313 doi:http://dx.doi.org/10.1093/emboj/18.20.5703

[3] Tenca P, Brotherton D, Montagnoli A, Rainoldi S, Albanese C, Santocanale C. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem. 2007 Jan 5;282(1):208-15. Epub 2006 Oct 24. PMID:17062569 doi:http://dx.doi.org/10.1074/jbc.M604457200

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4f9b is ON HOLD  until Paper Publication
+==Human CDC7 kinase in complex with DBF4 and PHA767491==
+<StructureSection load='4f9b' size='340' side='right'caption='[[4f9b]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-Authors: Hughes, S., Cherepanov, P.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4f9b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F9B FirstGlance]. <br>
-Description: Human CDC7 kinase in complex with DBF4 and PHA767491
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0SY:2-(PYRIDIN-4-YL)-1,5,6,7-TETRAHYDRO-4H-PYRROLO[3,2-C]PYRIDIN-4-ONE'>0SY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f9b OCA], [https://pdbe.org/4f9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f9b RCSB], [https://www.ebi.ac.uk/pdbsum/4f9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f9b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DBF4A_HUMAN DBF4A_HUMAN] Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.<ref>PMID:10373557</ref> <ref>PMID:10523313</ref> <ref>PMID:17062569</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Cherepanov P]]
+[[Category: Hughes S]]

4f9b: Difference between revisions

Latest revision as of 14:12, 1 March 2024

Human CDC7 kinase in complex with DBF4 and PHA767491Human CDC7 kinase in complex with DBF4 and PHA767491

Structural highlights

Function

References

Contents

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4f9b: Difference between revisions

Latest revision as of 14:12, 1 March 2024

Human CDC7 kinase in complex with DBF4 and PHA767491Human CDC7 kinase in complex with DBF4 and PHA767491

Structural highlights

Function

References

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