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==Structure of Ltp, a superinfection exclusion protein from the Streptococcus thermophilus temperate phage TP-J34==
==Structure of Ltp, a superinfection exclusion protein from the Streptococcus thermophilus temperate phage TP-J34==
<StructureSection load='4eqq' size='340' side='right' caption='[[4eqq]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
<StructureSection load='4eqq' size='340' side='right'caption='[[4eqq]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4eqq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_phage_tp-j34 Streptococcus phage tp-j34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQQ FirstGlance]. <br>
<table><tr><td colspan='2'>[[4eqq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_TP-J34 Streptococcus phage TP-J34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EQQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ltp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=73422 Streptococcus phage TP-J34])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eqq OCA], [http://pdbe.org/4eqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eqq RCSB], [http://www.ebi.ac.uk/pdbsum/4eqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eqq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eqq OCA], [https://pdbe.org/4eqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eqq RCSB], [https://www.ebi.ac.uk/pdbsum/4eqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eqq ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/O48388_9CAUD O48388_9CAUD]
Lipoproteins of temperate phage are a broad family of membrane proteins encoded in the lysogeny module of temperate phages. Expression of the ltpTP - J 34 gene of temperate Streptococcus thermophilus phage TP-J34 interferes with phage infection at the stage of triggering DNA release and injection into the cell. Here, we report the first structure of a superinfection exclusion protein. We have expressed and determined the X-ray structure of LtpTP - J 34 . The soluble domain of LtpTP - J 34 is composed of a tandem of three-helix helix-turn-helix (HTH) domains exhibiting a highly negatively charged surface. By isolating mutants of lactococcal phage P008wt with reduced sensitivities to LtpTP -J34 and by genome sequencing of such mutants we obtained evidence supporting the notion that LtpTP - J 34 targets the phage's tape measure protein (TMP) and blocks its insertion into the cytoplasmic membrane.
 
X-ray structure of a superinfection exclusion lipoprotein from phage TP-J34 and identification of the tape measure protein as its target.,Bebeacua C, Lorenzo Fajardo JC, Blangy S, Spinelli S, Bollmann S, Neve H, Cambillau C, Heller KJ Mol Microbiol. 2013 Jul;89(1):152-65. doi: 10.1111/mmi.12267. Epub 2013 Jun 7. PMID:23692331<ref>PMID:23692331</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4eqq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Streptococcus phage tp-j34]]
[[Category: Large Structures]]
[[Category: Bebeacua, C]]
[[Category: Streptococcus phage TP-J34]]
[[Category: Blangy, S]]
[[Category: Bebeacua C]]
[[Category: Cambillau,C]]
[[Category: Blangy S]]
[[Category: Heller,K]]
[[Category: Cambillau C]]
[[Category: Lorenzo, C]]
[[Category: Heller K]]
[[Category: Spinelli, S]]
[[Category: Lorenzo C]]
[[Category: Hth fold]]
[[Category: Spinelli S]]
[[Category: Lipoprotein ektodomain]]
[[Category: Protein binding]]
[[Category: Superinfection exclusion]]

Latest revision as of 14:04, 1 March 2024

Structure of Ltp, a superinfection exclusion protein from the Streptococcus thermophilus temperate phage TP-J34Structure of Ltp, a superinfection exclusion protein from the Streptococcus thermophilus temperate phage TP-J34

Structural highlights

4eqq is a 2 chain structure with sequence from Streptococcus phage TP-J34. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.05Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O48388_9CAUD

4eqq, resolution 2.05Å

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