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==A. fulgidus IscS-IscU complex structure==
==A. fulgidus IscS-IscU complex structure==
<StructureSection load='4eb7' size='340' side='right' caption='[[4eb7]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
<StructureSection load='4eb7' size='340' side='right'caption='[[4eb7]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4eb7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EB7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4eb7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EB7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4eb5|4eb5]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">iscS2, AF_0564 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU]), AF_0185, AF_0565 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eb7 OCA], [https://pdbe.org/4eb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eb7 RCSB], [https://www.ebi.ac.uk/pdbsum/4eb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eb7 ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eb7 OCA], [http://pdbe.org/4eb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eb7 RCSB], [http://www.ebi.ac.uk/pdbsum/4eb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eb7 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU]] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). [[http://www.uniprot.org/uniprot/ISCU1_ARCFU ISCU1_ARCFU]] A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
[https://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron-sulfur cluster assembly. The X-ray structure of an anaerobically produced, mutated (Fe(2) S(2) -(IscS-IscU(D35A) ))(2) complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine. In air-exposed crystals the cluster is oxidized to an Fe(2) S-S center; D35 is essential for complex dissociation.
 
(IscS-IscU)(2) Complex Structures Provide Insights into Fe(2) S(2) Biogenesis and Transfer.,Marinoni EN, de Oliveira JS, Nicolet Y, Raulfs EC, Amara P, Dean DR, Fontecilla-Camps JC Angew Chem Int Ed Engl. 2012 Apr 18. doi: 10.1002/anie.201201708. PMID:22511353<ref>PMID:22511353</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4eb7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Arcfu]]
[[Category: Archaeoglobus fulgidus DSM 4304]]
[[Category: Cysteine desulfurase]]
[[Category: Large Structures]]
[[Category: Amara, P]]
[[Category: Amara P]]
[[Category: Dean, D R]]
[[Category: Dean DR]]
[[Category: Fontecilla-Camps, J C]]
[[Category: Fontecilla-Camps JC]]
[[Category: Marinoni, E N]]
[[Category: Marinoni EN]]
[[Category: Nicolet, Y]]
[[Category: Nicolet Y]]
[[Category: Oliveira, J S.de]]
[[Category: Raulfs EC]]
[[Category: Raulfs, E C]]
[[Category: De Oliveira JS]]
[[Category: Transferase-metal binding protein complex]]

Latest revision as of 14:02, 1 March 2024

A. fulgidus IscS-IscU complex structureA. fulgidus IscS-IscU complex structure

Structural highlights

4eb7 is a 3 chain structure with sequence from Archaeoglobus fulgidus DSM 4304. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.75Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ISCS2_ARCFU Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).

4eb7, resolution 2.75Å

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