4e6b: Difference between revisions

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<StructureSection load='4e6b' size='340' side='right'caption='[[4e6b]], [[Resolution|resolution]] 1.47&Aring;' scene=''>
<StructureSection load='4e6b' size='340' side='right'caption='[[4e6b]], [[Resolution|resolution]] 1.47&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4e6b]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6B FirstGlance]. <br>
<table><tr><td colspan='2'>[[4e6b]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E6B FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e48|4e48]], [[4e5c|4e5c]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.47&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6b OCA], [http://pdbe.org/4e6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e6b RCSB], [http://www.ebi.ac.uk/pdbsum/4e6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6b ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6b OCA], [https://pdbe.org/4e6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e6b RCSB], [https://www.ebi.ac.uk/pdbsum/4e6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6b ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human genetic trinucleotide repeat expansion diseases (TREDs) are characterized by triplet repeat expansions, most frequently found as CNG-tracts in genome. At RNA level, such expansions suggestively result in formation of double-helical hairpins that become a potential source for small RNAs involved in RNA interference (RNAi). Here, we present three crystal structures of RNA fragments composed of triplet repeats CUG and CGG/CUG, as well as two crystal structures of same triplets in a protein-bound state. We show that both 20mer pG(CUG)(6)C and 19mer pGG(CGG)(3)(CUG)(2)CC form A-RNA duplexes, in which U.U or G.U mismatches are flanked/stabilized by two consecutive Watson-Crick G.C base pairs resulting in high-stacking GpC steps in every third position of the duplex. Despite interruption of this regularity in another 19mer, p(CGG)(3)C(CUG)(3), the oligonucleotide still forms regular double-helical structure, characterized, however, by 12 bp (rather than 11 bp) per turn. Analysis of newly determined molecular structures reveals the dynamic aspects of U.U and G.U mismatching within CNG-repetitive A-RNA and in a protein-bound state, as well as identifies an additional mode of U.U pairing essential for its dynamics and sheds the light on possible role of regularity of trinucleotide repeats for double-helical RNA structure. Findings are important for understanding the structural behavior of CNG-repetitive RNA double helices implicated in TREDs.
Structural dynamics of double-helical RNAs composed of CUG/CUG- and CUG/CGG-repeats.,Tamjar J, Katorcha E, Popov A, Malinina L J Biomol Struct Dyn. 2012 Jun 25. PMID:22731704<ref>PMID:22731704</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4e6b" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Malinina, L]]
[[Category: Malinina L]]
[[Category: Popov, A]]
[[Category: Popov A]]
[[Category: Tamjar, J]]
[[Category: Tamjar J]]
[[Category: Rna]]
[[Category: Sirna]]
[[Category: Trinucleotide repeat expansion]]

Latest revision as of 14:02, 1 March 2024

Crystal Structure of statistically disordered 19mer duplex p(CGG)3C(CUG)3Crystal Structure of statistically disordered 19mer duplex p(CGG)3C(CUG)3

Structural highlights

4e6b is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.47Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

4e6b, resolution 1.47Å

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