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==Crystal Structure of Papaya mosaic virus capsid protein==
==Crystal Structure of Papaya mosaic virus capsid protein==
<StructureSection load='4dox' size='340' side='right' caption='[[4dox]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='4dox' size='340' side='right'caption='[[4dox]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4dox]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pmv Pmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DOX FirstGlance]. <br>
<table><tr><td colspan='2'>[[4dox]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Papaya_mosaic_virus Papaya mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DOX FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PapMVgp5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12181 PMV])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dox OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dox RCSB], [http://www.ebi.ac.uk/pdbsum/4dox PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dox OCA], [https://pdbe.org/4dox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dox RCSB], [https://www.ebi.ac.uk/pdbsum/4dox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dox ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/CAPSD_PMV CAPSD_PMV] Required for genome encapsidation. Forms ribonucleoprotein complexes along with TGB1 helicase and viral RNA.
Papaya mosaic virus (PapMV) is a filamentous plant virus that belongs to the Alphaflexiviridae family. Flexible filamentous viruses have defied more than two decades of effort in fiber diffraction, and no high-resolution structure is available for any member of the Alphaflexiviridae family. Here, we report our structural characterization of PapMV by X-ray crystallography and cryo-electron microscopy three-dimensional reconstruction. We found that PapMV is 135A in diameter with a helical symmetry of ~10 subunits per turn. Crystal structure of the C-terminal truncated PapMV coat protein (CP) reveals a novel all-helix fold with seven alpha-helices. Thus, the PapMVCP structure is different from the four-helix-bundle fold of tobacco mosaic virus in which helix bundling dominates the subunit interface in tobacco mosaic virus and conveys rigidity to the rod virus. PapMV CP was crystallized as an asymmetrical dimer in which one protein lassoes the other by the N-terminal peptide. Mutation of residues critical to the inter-subunit lasso interaction abolishes CP polymerization. The crystal structure suggests that PapMV may polymerize via the consecutive N-terminal loop lassoing mechanism. The structure of PapMV will be useful for rational design and engineering of the PapMV nanoparticles into innovative vaccines.
 
Crystal Structure of the Coat Protein of the Flexible Filamentous Papaya Mosaic Virus.,Yang S, Wang T, Bohon J, Gagne ME, Bolduc M, Leclerc D, Li H J Mol Biol. 2012 May 30. PMID:22659319<ref>PMID:22659319</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pmv]]
[[Category: Large Structures]]
[[Category: Li, H]]
[[Category: Papaya mosaic virus]]
[[Category: Wang, T]]
[[Category: Li H]]
[[Category: All helix capsid protein]]
[[Category: Wang T]]
[[Category: Viral protein]]
[[Category: Virus capsid structure]]

Latest revision as of 13:53, 1 March 2024

Crystal Structure of Papaya mosaic virus capsid proteinCrystal Structure of Papaya mosaic virus capsid protein

Structural highlights

4dox is a 2 chain structure with sequence from Papaya mosaic virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPSD_PMV Required for genome encapsidation. Forms ribonucleoprotein complexes along with TGB1 helicase and viral RNA.

4dox, resolution 2.70Å

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OCA
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