3ven: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(6 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:3ven.jpg|left|200px]]


<!--
==Crystal structure of the O-carbamoyltransferase TobZ==
The line below this paragraph, containing "STRUCTURE_3ven", creates the "Structure Box" on the page.
<StructureSection load='3ven' size='340' side='right'caption='[[3ven]], [[Resolution|resolution]] 1.57&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3ven]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptoalloteichus_tenebrarius Streptoalloteichus tenebrarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VEN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VEN FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.57&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
{{STRUCTURE_3ven|  PDB=3ven  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ven FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ven OCA], [https://pdbe.org/3ven PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ven RCSB], [https://www.ebi.ac.uk/pdbsum/3ven PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ven ProSAT]</span></td></tr>
 
</table>
===Crystal structure of the O-carbamoyltransferase TobZ===
== Function ==
 
[https://www.uniprot.org/uniprot/TOBZ_STRSD TOBZ_STRSD] TobZ is involved in the biosynthesis of the 2-deoxystreptamine-containing aminoglycoside antibiotics such as nebramycin 5 and 6-O-carbamoylkanamycin. Catalyzes the hydrolysis of carbamoyl phosphate and its subsequent adenylation by ATP to yield O-carbamoyladenylate. Then it catalyzes the transfer of the carbamoyl moiety from O-carbamoyladenylate to the tobramycin 6-hydroxy group to yield nebramycin 5. It catalyzes the same reaction with kanamycin A. These reactions are considerably slower in the presence of deoxy-ATP.<ref>PMID:20936279</ref> <ref>PMID:22383337</ref>
 
== References ==
==About this Structure==
<references/>
[[3ven]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptoalloteichus_tenebrarius Streptoalloteichus tenebrarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VEN OCA].  
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptoalloteichus tenebrarius]]
[[Category: Streptoalloteichus tenebrarius]]
[[Category: Goerlich, S.]]
[[Category: Goerlich S]]
[[Category: Jaenecke, F.]]
[[Category: Jaenecke F]]
[[Category: Parthier, C.]]
[[Category: Parthier C]]
[[Category: Stubbs, M T.]]
[[Category: Stubbs MT]]
[[Category: Adenylation]]
[[Category: Antibiotic biosynthesis]]
[[Category: Enzyme evolution]]
[[Category: Structural enzymology]]
[[Category: Substrate assisted catalysis]]
[[Category: Substrate channeling]]
[[Category: Transferase]]

Latest revision as of 13:35, 1 March 2024

Crystal structure of the O-carbamoyltransferase TobZCrystal structure of the O-carbamoyltransferase TobZ

Structural highlights

3ven is a 1 chain structure with sequence from Streptoalloteichus tenebrarius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.57Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TOBZ_STRSD TobZ is involved in the biosynthesis of the 2-deoxystreptamine-containing aminoglycoside antibiotics such as nebramycin 5 and 6-O-carbamoylkanamycin. Catalyzes the hydrolysis of carbamoyl phosphate and its subsequent adenylation by ATP to yield O-carbamoyladenylate. Then it catalyzes the transfer of the carbamoyl moiety from O-carbamoyladenylate to the tobramycin 6-hydroxy group to yield nebramycin 5. It catalyzes the same reaction with kanamycin A. These reactions are considerably slower in the presence of deoxy-ATP.[1] [2]

References

  1. ↑ Ni X, Li D, Yang L, Huang T, Li H, Xia H. Construction of kanamycin B overproducing strain by genetic engineering of Streptomyces tenebrarius. Appl Microbiol Biotechnol. 2011 Feb;89(3):723-31. doi: 10.1007/s00253-010-2908-5., Epub 2010 Oct 9. PMID:20936279 doi:http://dx.doi.org/10.1007/s00253-010-2908-5
  2. ↑ Parthier C, Gorlich S, Jaenecke F, Breithaupt C, Brauer U, Fandrich U, Clausnitzer D, Wehmeier UF, Bottcher C, Scheel D, Stubbs MT. The O-Carbamoyltransferase TobZ Catalyzes an Ancient Enzymatic Reaction. Angew Chem Int Ed Engl. 2012 Mar 1. doi: 10.1002/anie.201108896. PMID:22383337 doi:10.1002/anie.201108896

3ven, resolution 1.57Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3ven&oldid=4072733"