3tsy: Difference between revisions

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==4-Coumaroyl-CoA Ligase::Stilbene Synthase fusion protein==
==4-Coumaroyl-CoA Ligase::Stilbene Synthase fusion protein==
<StructureSection load='3tsy' size='340' side='right' caption='[[3tsy]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='3tsy' size='340' side='right'caption='[[3tsy]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3tsy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TSY FirstGlance]. <br>
<table><tr><td colspan='2'>[[3tsy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TSY FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3a9u|3a9u]], [[3a9v|3a9v]], [[1xes|1xes]], [[1z1e|1z1e]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">4CL1, At1g51680, At4CL1, F19C24.11, VvSTS, STS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsy OCA], [https://pdbe.org/3tsy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tsy RCSB], [https://www.ebi.ac.uk/pdbsum/3tsy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tsy ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tsy RCSB], [http://www.ebi.ac.uk/pdbsum/3tsy PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/4CL1_ARATH 4CL1_ARATH] Produces CoA thioesters of a variety of hydroxy- and methoxy-substituted cinnamic acids, which are used to synthesize several phenylpropanoid-derived compounds, including anthocyanins, flavonoids, isoflavonoids, coumarins, lignin, suberin and wall-bound phenolics.[https://www.uniprot.org/uniprot/Q2HY10_VITVI Q2HY10_VITVI]
To increase the biochemical efficiency of biosynthetic systems, metabolic engineers have explored different approaches for organizing enzymes, including the generation of unnatural fusion proteins. Previous work aimed at improving the biosynthesis of resveratrol, a stilbene associated a range of health-promoting activities, in yeast used an unnatural engineered fusion protein of Arabidopsis thaliana (thale cress) 4-coumaroyl-CoA ligase (At4CL1) and Vitis vinifera (grape) stilbene synthase (VvSTS) to increase resveratrol levels 15-fold relative to yeast expressing the individual enzymes. Here we present the crystallographic and biochemical analysis of the 4CL::STS fusion protein. Determination of the X-ray crystal structure of 4CL::STS provides the first molecular view of an artificial didomain adenylation/ketosynthase fusion protein. Comparison of the steady-state kinetic properties of At4CL1, VvSTS, and 4CL::STS demonstrates that the fusion protein improves catalytic efficiency of either reaction less than 3-fold. Structural and kinetic analysis suggests that colocalization of the two enzyme active sites within 70 A of each other provides the basis for enhanced in vivo synthesis of resveratrol.
 
Structural and Kinetic Analysis of the Unnatural Fusion Protein 4-Coumaroyl-CoA Ligase::Stilbene Synthase.,Wang Y, Yi H, Wang M, Yu O, Jez JM J Am Chem Soc. 2011 Dec 5. PMID:22129213<ref>PMID:22129213</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Arath]]
[[Category: Arabidopsis thaliana]]
[[Category: Jez, J M]]
[[Category: Large Structures]]
[[Category: Yi, H]]
[[Category: Vitis vinifera]]
[[Category: Fusion protein]]
[[Category: Jez JM]]
[[Category: Ligase]]
[[Category: Yi H]]
[[Category: Transferase]]

Latest revision as of 13:11, 1 March 2024

4-Coumaroyl-CoA Ligase::Stilbene Synthase fusion protein4-Coumaroyl-CoA Ligase::Stilbene Synthase fusion protein

Structural highlights

3tsy is a 1 chain structure with sequence from Arabidopsis thaliana and Vitis vinifera. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.1Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

4CL1_ARATH Produces CoA thioesters of a variety of hydroxy- and methoxy-substituted cinnamic acids, which are used to synthesize several phenylpropanoid-derived compounds, including anthocyanins, flavonoids, isoflavonoids, coumarins, lignin, suberin and wall-bound phenolics.Q2HY10_VITVI

3tsy, resolution 3.10Å

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