3t5q: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
Line 3: Line 3:
<StructureSection load='3t5q' size='340' side='right'caption='[[3t5q]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
<StructureSection load='3t5q' size='340' side='right'caption='[[3t5q]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>
<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mopeia_Lassa_virus_reassortant_29 Mopeia Lassa virus reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t5n|3t5n]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [https://pdbe.org/3t5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [https://www.ebi.ac.uk/pdbsum/3t5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5q ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [https://pdbe.org/3t5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [https://www.ebi.ac.uk/pdbsum/3t5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5q ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/Q5S585_9VIRU Q5S585_9VIRU]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]  
[https://www.uniprot.org/uniprot/Q5S585_9VIRU Q5S585_9VIRU] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Arenaviruses cause disease in industrialized and developing nations alike. Among them, the hemorrhagic fever virus Lassa is responsible for ~300,000-500,000 infections/y in Western Africa. The arenavirus nucleoprotein (NP) forms the protein scaffold of the genomic ribonucleoprotein complexes and is critical for transcription and replication of the viral genome. Here, we present crystal structures of the RNA-binding domain of Lassa virus NP in complex with ssRNA. This structure shows, in contrast to the predicted model, that RNA binds in a deep, basic crevice located entirely within the N-terminal domain. Furthermore, the NP-ssRNA structures presented here, combined with hydrogen-deuterium exchange/MS and functional studies, suggest a gating mechanism by which NP opens to accept RNA. Directed mutagenesis and functional studies provide a unique look into how the arenavirus NPs bind to and protect the viral genome and also suggest the likely assembly by which viral ribonucleoprotein complexes are organized.
 
Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding.,Hastie KM, Liu T, Li S, King LB, Ngo N, Zandonatti MA, Woods VL Jr, de la Torre JC, Saphire EO Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19365-70. Epub 2011 Nov 14. PMID:22084115<ref>PMID:22084115</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3t5q" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mopeia lassa reassortant 29]]
[[Category: Mopeia Lassa virus reassortant 29]]
[[Category: Hastie, K M]]
[[Category: Hastie KM]]
[[Category: King, L B]]
[[Category: King LB]]
[[Category: Liu, T]]
[[Category: Liu T]]
[[Category: Ngo, N]]
[[Category: Ngo N]]
[[Category: Saphire, E O]]
[[Category: Saphire EO]]
[[Category: Torre, J C.de la]]
[[Category: Woods VL]]
[[Category: Woods, V L]]
[[Category: Zandonatti MA]]
[[Category: Zandonatti, M A]]
[[Category: De la Torre JC]]
[[Category: Single stranded rna]]
[[Category: Ssrna]]
[[Category: Viral protein-rna complex]]

Latest revision as of 13:03, 1 March 2024

3A structure of Lassa virus nucleoprotein in complex with ssRNA3A structure of Lassa virus nucleoprotein in complex with ssRNA

Structural highlights

3t5q is a 12 chain structure with sequence from Escherichia coli and Mopeia Lassa virus reassortant 29. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q5S585_9VIRU Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]

See Also

3t5q, resolution 3.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3t5q&oldid=4071722"