3s4l: Difference between revisions

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-[[Image:3s4l.jpg|left|200px]]
-<!--
+==The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii==
-The line below this paragraph, containing "STRUCTURE_3s4l", creates the "Structure Box" on the page.
+<StructureSection load='3s4l' size='340' side='right'caption='[[3s4l]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3s4l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3m5f 3m5f]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S4L FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-{{STRUCTURE_3s4l|  PDB=3s4l  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s4l OCA], [https://pdbe.org/3s4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s4l RCSB], [https://www.ebi.ac.uk/pdbsum/3s4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s4l ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CS3HD_METJA CS3HD_METJA] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a ssDNA and ssRNA nuclease, with both endo- and 3' to 5' exonuclease activities, acting on substrates with free single-stranded 3' ends. Double-stranded nucleic acids are not substrates. Activity is higher for DNA than RNA. Templates include R-loops (a bubble-like structure formed when ssRNA replaces one strand in a dsDNA, such as crRNA is thought to form with CRISPR target DNA), circular ssDNA, 2',3'-cAMP and 2',3'-cGMP. Probably generates 3'-phosphate and 5'-hydroxyl ends. In the presence of the Cas3 helicase and ATP dsDNA templates are also degraded.<ref>PMID:22009198</ref>
-===The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii===
+==See Also==
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-[[3s4l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3m5f 3m5f]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S4L OCA].
+__TOC__
-[[Category: Methanocaldococcus jannaschii]]
+</StructureSection>
-[[Category: Brown, G.]]
+[[Category: Large Structures]]
-[[Category: Edwards, A.]]
+[[Category: Methanocaldococcus jannaschii DSM 2661]]
-[[Category: MCSG, Midwest Center for Structural Genomics.]]
+[[Category: Brown G]]
-[[Category: Petit, P.]]
+[[Category: Edwards A]]
-[[Category: Savchenko, A.]]
+[[Category: Joachimiak A]]
-[[Category: Yakunin, A.]]
+[[Category: Petit P]]
-[[Category: Hd-motif]]
+[[Category: Savchenko A]]
-[[Category: Hydrolase]]
+[[Category: Yakunin A]]
-[[Category: Immune system]]
-[[Category: Mcsg]]
-[[Category: Midwest center for structural genomic]]
-[[Category: Nuclease]]
-[[Category: Psi-biology]]
-[[Category: Structural genomic]]