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| ==The Crystal Structure of Leishmania major Peroxidase== | | ==The Crystal Structure of Leishmania major Peroxidase== |
| <StructureSection load='3riv' size='340' side='right' caption='[[3riv]], [[Resolution|resolution]] 1.76Å' scene=''> | | <StructureSection load='3riv' size='340' side='right'caption='[[3riv]], [[Resolution|resolution]] 1.76Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3riv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIV FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3riv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major_strain_Friedlin Leishmania major strain Friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIV FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3riw|3riw]]</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">APX, LMJF_34_0070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3riv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riv OCA], [https://pdbe.org/3riv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3riv RCSB], [https://www.ebi.ac.uk/pdbsum/3riv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3riv ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11] </span></td></tr> | |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3riv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riv OCA], [http://pdbe.org/3riv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3riv RCSB], [http://www.ebi.ac.uk/pdbsum/3riv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3riv ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q4Q3K2_LEIMA Q4Q3K2_LEIMA] |
| The parasitic protozoa, Leishmania major, produces a peroxidase (abbreviated LmP) that exhibits activities characteristic of both yeast cytochrome c peroxidase (CCP) and plant cytosolic ascorbate peroxidase (APX). One common feature is a key Trp residue, Trp208 in LmP and Trp191 in CCP, that is situated adjacent to the proximal His heme ligand in CCP, APX and LmP. In CCP Trp191 forms a stable cationic radical after reaction with H2O2 to form Compound I; in APX the radical is located on the porphyrin ring. In order to clarify the role of Trp208 in LmP and to further probe peroxidase structure-function relationships we have determined the crystal structure of LmP and have studied the role of Trp208 using electron paramagnetic resonance spectroscopy (EPR), mutagenesis, and enzyme kinetics. Both CCP and LmP have an extended section of beta structure near Trp191 and 208, respectively, which is absent in APX. This region provides stability to the Trp191 radical in CCP. EPR of LmP Compound I exhibits an intense and stable signal similar to CCP Compound I. In the LmP Trp208Phe mutant this signal disappears indicating that Trp208 forms a stable cationic radical. In LmP conversion of the Cys197 to Thr significantly weakens the Compound I EPR signal and dramatically lowers enzyme activity. These results further support the view that modulation of the local electrostatic environment controls the stability of the Trp radical in peroxidases. Our results also suggest that the biological role of LmP is to function as a cytochrome c peroxdase.
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| The crystal structure of Leishmania major peroxidase and characterization of the compound I tryptophan radical.,Jasion VS, Polanco JA, Meharenna YT, Li H, Poulos TL J Biol Chem. 2011 May 13. PMID:21566139<ref>PMID:21566139</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3riv" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Ascorbate peroxidase|Ascorbate peroxidase]] | | *[[Ascorbate peroxidase 3D structures|Ascorbate peroxidase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: L-ascorbate peroxidase]] | | [[Category: Large Structures]] |
| [[Category: Leishmania major mhom/il/81/friedlin]] | | [[Category: Leishmania major strain Friedlin]] |
| [[Category: Jasion, V S]] | | [[Category: Jasion VS]] |
| [[Category: Li, H]] | | [[Category: Li H]] |
| [[Category: Poulos, T L]] | | [[Category: Poulos TL]] |
| [[Category: Alpha helical bundle]]
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| [[Category: Heme peroxidase]]
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| [[Category: Oxidoreductase]]
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