3rio: Difference between revisions

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[[Image:3rio.png|left|200px]]


{{STRUCTURE_3rio|  PDB=3rio  |  SCENE=  }}
==Crystal structure of GlcT CAT-PRDI==
 
<StructureSection load='3rio' size='340' side='right'caption='[[3rio]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
===Crystal structure of GlcT CAT-PRDI===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3rio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIO FirstGlance]. <br>
{{ABSTRACT_PUBMED_22750856}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rio OCA], [https://pdbe.org/3rio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rio RCSB], [https://www.ebi.ac.uk/pdbsum/3rio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rio ProSAT]</span></td></tr>
[[3rio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIO OCA].  
</table>
 
== Function ==
==Reference==
[https://www.uniprot.org/uniprot/GLCT_BACSU GLCT_BACSU] Mediates the positive regulation of the glucose PTS operon (ptsGHI) by functioning as an antiterminator factor of transcription via its interaction with the RNA-antiterminator (RAT) sequence located upstream of the ptsG gene. The RNA-binding domain of GlcT directly binds to the RNA antiterminator (RAT) sequence and prevents transcriptional termination. GlcT binding requires two identical and nearly symmetrical triple base pairings in the RAT sequence.<ref>PMID:11902727</ref> <ref>PMID:9765562</ref>
<ref group="xtra">PMID:022750856</ref><references group="xtra"/>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Becker, S.]]
[[Category: Large Structures]]
[[Category: Grosse, C.]]
[[Category: Becker S]]
[[Category: Himmel, S.]]
[[Category: Grosse C]]
[[Category: Wolff, S.]]
[[Category: Himmel S]]
[[Category: Four helix bundle]]
[[Category: Wolff S]]
[[Category: Transcription]]
[[Category: Transcriptional antitermination]]
[[Category: Twisted beta sheet]]

Latest revision as of 12:43, 1 March 2024

Crystal structure of GlcT CAT-PRDICrystal structure of GlcT CAT-PRDI

Structural highlights

3rio is a 1 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.99Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLCT_BACSU Mediates the positive regulation of the glucose PTS operon (ptsGHI) by functioning as an antiterminator factor of transcription via its interaction with the RNA-antiterminator (RAT) sequence located upstream of the ptsG gene. The RNA-binding domain of GlcT directly binds to the RNA antiterminator (RAT) sequence and prevents transcriptional termination. GlcT binding requires two identical and nearly symmetrical triple base pairings in the RAT sequence.[1] [2]

References

  1. ↑ Stulke J, Martin-Verstraete I, Zagorec M, Rose M, Klier A, Rapoport G. Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT. Mol Microbiol. 1997 Jul;25(1):65-78. PMID:11902727
  2. ↑ Bachem S, Stulke J. Regulation of the Bacillus subtilis GlcT antiterminator protein by components of the phosphotransferase system. J Bacteriol. 1998 Oct;180(20):5319-26. PMID:9765562

3rio, resolution 1.99Å

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