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New page: '''Unreleased structure''' The entry 8ik4 is ON HOLD Authors: Adhav, V.A., Saikrishnan, K. Description: Structure of DNA binding domain of McrBC endonuclease bound to hemimethylated DN... |
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==Structure of DNA binding domain of McrBC endonuclease bound to hemimethylated DNA: L68F mutant== | |||
<StructureSection load='8ik4' size='340' side='right'caption='[[8ik4]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8ik4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/DNA_molecule DNA molecule] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IK4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr> | ||
[[Category: | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ik4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ik4 OCA], [https://pdbe.org/8ik4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ik4 RCSB], [https://www.ebi.ac.uk/pdbsum/8ik4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ik4 ProSAT]</span></td></tr> | ||
[[Category: | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/MCRB_ECOLI MCRB_ECOLI] Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities. | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: DNA molecule]] | |||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | |||
[[Category: Adhav VA]] | |||
[[Category: Saikrishnan K]] | |||
Latest revision as of 12:37, 1 March 2024
Structure of DNA binding domain of McrBC endonuclease bound to hemimethylated DNA: L68F mutantStructure of DNA binding domain of McrBC endonuclease bound to hemimethylated DNA: L68F mutant
Structural highlights
FunctionMCRB_ECOLI Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities. |
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